Anti-Rub1 antibody, generated by immunization with full-length, recombinant yeast Rub1, was tested by western blot against a yeast cell lysate. A dilution of the antibody between 1:200 and 1:1,000 will show strong reactivity specifically with free Rub1 protein (indicated by arrow) and Rub1 conjugates. In this blot, the antibody was used at a 1:500 dilution and was incubated overnight at 4o C in 5% non-fat dry milk in TBST. Detection occurred using a 1:2000 dilution of HRP-labeled Donkey anti-Rabbit IgG
Anti-Rub1 antibody, generated by immunization with full-length, recombinant yeast Rub1, was tested by western blot against a yeast cell lysate. A dilution of the antibody between 1:200 and 1:1,000 will show strong reactivity specifically with free Rub1 protein (indicated by arrow) and Rub1 conjugates. In this blot, the antibody was used at a 1:500 dilution and was incubated overnight at 4o C in 5% non-fat dry milk in TBST. Detection occurred using a 1:2000 dilution of HRP-labeled Donkey anti-Rabbit IgG
Rub1 antibody
GTX48823
Overview
- SupplierGeneTex
- Product NameRub1 antibody
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:2000. ELISA: 1:1000-1:5000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot, ELISA
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID851717
- Target nameRUB1
- Target descriptionNEDD8 family protein RUB1
- Target synonymsNEDD8 family protein RUB1; YDR139C
- HostRabbit
- IsotypeIgG
- Protein IDQ03919
- Protein NameNEDD8-like protein RUB1
- ReactivityYeast
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203