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Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA751157LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA751157LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA751157LA01HU at dilution of 1:100

RUFY4 Antibody

CSB-PA751157LA01HU
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetRUFY4
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Overview

  • Supplier
    Cusabio
  • Product Name
    RUFY4 Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID285180
  • Target name
    RUFY4
  • Target description
    RUN and FYVE domain containing 4
  • Target synonyms
    ZFYVE31, RUN and FYVE domain-containing protein 4
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ6ZNE9
  • Protein Name
    RUN and FYVE domain-containing protein 4
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of RUFY4 using anti-RUFY4 antibody (A16707-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: monkey COS-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RUFY4 antigen affinity purified polyclonal antibody (Catalog # A16707-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RUFY4 at approximately 64 kDa. The expected band size for RUFY4 is at 64 kDa.
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Immunohistochemical staining of human duodenum shows moderate cytoplasmic positivity in glandular cells.
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