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ProductsBack/RUNX2 Polyclonal Antibody
Lane 1: HL60 cell lysates; Lane 2: Mouse testis lysates probed with RUNX2 Polyclonal Antibody, Unconjugated (bs-1134R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: HL60 cell lysates; Lane 2: Mouse testis lysates probed with RUNX2 Polyclonal Antibody, Unconjugated (bs-1134R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: HL60 cell lysates; Lane 2: Mouse testis lysates probed with RUNX2 Polyclonal Antibody, Unconjugated (bs-1134R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

RUNX2 Polyclonal Antibody

BS-1134R
Bioss
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityBovine, Canine, Chicken, Equine, Human, Mouse, Porcine, Rabbit, Rat, Sheep
TargetRUNX2
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Overview

  • Supplier
    Bioss
  • Product Name
    RUNX2 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID860
  • Target name
    RUNX2
  • Target description
    RUNX family transcription factor 2
  • Target synonyms
    AML3, CBF-alpha-1, CBFA1, CCD, CCD1, CLCD, OSF-2, OSF2, PEA2aA, PEBP2aA, runt-related transcription factor 2, PEA2-alpha A, PEBP2-alpha A, SL3-3 enhancer factor 1 alpha A subunit, SL3/AKV core-binding factor alpha A subunit, acute myeloid leukemia 3 protein, core-binding factor, runt domain, alpha subunit 1, oncogene AML-3, osteoblast-specific transcription factor 2, polyomavirus enhancer-binding protein 2 alpha A subunit, runt related transcription factor 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ13950
  • Protein Name
    Runt-related transcription factor 2
  • Reactivity
    Bovine, Canine, Chicken, Equine, Human, Mouse, Porcine, Rabbit, Rat, Sheep
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

References

  • Structure and ingredient-based biomimetic scaffolds combining with autologous bone marrow-derived mesenchymal stem cell sheets for bone-tendon healing. Tang Y et al., 2020 May, Biomaterials
    Read this paper
  • Sulfonated chitosan oligosaccharide alleviates the inhibitory effect of basic fibroblast growth factor on osteogenic differentiation of human periodontal ligament stem cells. Li Y et al., 2020 Jul, J Periodontol
    Read this paper
  • Silver nanoparticles promote osteogenic differentiation of human periodontal ligament fibroblasts by regulating the RhoA-TAZ axis. Xu Y et al., 2019 Aug, Cell Biol Int
    Read this paper
  • Modulation of proliferation and differentiation of gingiva-derived mesenchymal stem cells by concentrated growth factors: Potential implications in tissue engineering for dental regeneration and repair. Chen X et al., 2019 Jul, Int J Mol Med
    Read this paper
  • Comparative Evaluation of the Book-Type Acellular Bone Scaffold and Fibrocartilage Scaffold for Bone-Tendon Healing. Lu H et al., 2019 Apr 12, J Orthop Res
    Read this paper
  • The role of inhibition by phosphocitrate and its analogue in chondrocyte differentiation and subchondral bone advance in Hartley guinea pigs. Sun Y et al., 2018 Apr, Exp Ther Med
    Read this paper
  • Blockade of hypoxia-induced CXCR4 with AMD3100 inhibits production of OA-associated catabolic mediators IL-1beta and MMP-13. Li P et al., 2016 Aug, Mol Med Rep
    Read this paper
  • Angiogenesis and bone regeneration by allogeneic mesenchymal stem cell intravenous transplantation in rabbit model of avascular necrotic femoral head. Li Z et al., 2013 Jul, J Surg Res
    Read this paper

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Figure 1. Western blot analysis of RUNX2 using anti-RUNX2 antibody (PB9158). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: recombinant human RUNX2 protein 0.5 ng. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RUNX2 antigen affinity purified polyclonal antibody (Catalog # PB9158) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RUNX2 at approximately 50 kDa. The expected band size for RUNX2 is at 50 kDa.
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