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RUVBL1 antibody [HL3323]

GTX641088
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetRUVBL1
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Overview

  • Supplier
    GeneTex
  • Product Name
    RUVBL1 antibody [HL3323]
  • Delivery Days Customer
    7
  • Application Supplier Note
    WB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    HL3323
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID8607
  • Target name
    RUVBL1
  • Target description
    RuvB like AAA ATPase 1
  • Target synonyms
    49 kDa TATA box-binding protein-interacting protein; 49 kDa TBP-interacting protein; 54 kDa erythrocyte cytosolic protein; ECP54; ECP-54; epididymis secretory sperm binding protein; INO80 complex subunit H; INO80H; NMP 238; NMP238; nuclear matrix protein 238; PONTIN; pontin 52; Pontin52; RuvB (E coli homolog)-like 1; ruvB-like 1; RuvB-like AAA ATPase; RVB1; TAP54-alpha; TATA binding protein interacting protein 49 kDa; TIH1; TIP49; TIP49A; TIP60-associated protein 54-alpha
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9Y265
  • Protein Name
    RuvB-like 1
  • Scientific Description
    This gene encodes a protein that has both DNA-dependent ATPase and DNA helicase activities and belongs to the ATPases associated with diverse cellular activities (AAA+) protein family. The encoded protein associates with several multisubunit transcriptional complexes and with protein complexes involved in both ATP-dependent remodeling and histone modification. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TIP49A/RUVBL1 using anti-TIP49A/RUVBL1 antibody (A02049-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human HT1080 whole cell lysates, Lane 4: monkey COS-7 whole cell lysates, Lane 5: human MCF-7 whole cell lysates, Lane 6: human Daudi whole cell lysates, Lane 7: human MOLT-4 whole cell lysates, Lane 8: human HEL whole cell lysates, Lane 9: rat testis tissue lysates, Lane 10: rat C6 whole cell lysates, Lane 11: mouse testis tissue lysates, Lane 12: mouse NIH/3T3 whole cell lysates. red to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TIP49A/RUVBL1 antigen affinity purified polyclonal antibody (Catalog # A02049-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TIP49A/RUVBL1 at approximately 54 kDa. The expected band size for TIP49A/RUVBL1 is at 50 kDa.
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