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ProductsBack/RXTA / RXR-Alpha Antibody

RXTA / RXR-Alpha Antibody

LS-C760919
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetRXRA
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    RXTA / RXR-Alpha Antibody
  • Delivery Days Customer
    14
  • Application Supplier Note
    Applications should be user optimized.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:500 - 1:1000) Applications should be user optimized.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID6256
  • Target name
    RXRA
  • Target description
    retinoid X receptor alpha
  • Target synonyms
    NR2B1; nuclear receptor subfamily 2 group B member 1; retinoic acid receptor RXR-alpha; retinoid X nuclear receptor alpha
  • Host
    Rabbit
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of RXRA using anti-RXRA antibody (A01299-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human COLO-320 whole cell lysates, Lane 3: human A431 whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: rat heart tissue lysates, Lane 6: mouse heart tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RXRA antigen affinity purified polyclonal antibody (Catalog # A01299-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RXRA at approximately 51-55KD. The expected band size for RXRA is at 51KD.
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