S1 Nuclease

Cosmo Bio USA

S1 Nuclease

16

Research Use Only

S1 Nuclease is a single-stranded-specific endonuclease that hydrolyzes single-stranded DNA and RNA into acid-soluble 5- mononucleotides. It hydrolyzes single-stranded regions in duplex DNA such as loops and gaps. This S1 Nuclease is from Aspergillus sp. SPECIFICATIONS: Storage: 30mM sodium acetate (pH4.6), 50mM NaCl, 1M ZnCl2, 50% glycerol Activity: ≥100 units/microl Molecular Weight: 32,000 glycoprotein containing Zn2+ Optimum pH and temperature: See Figure pH and thermal stability: See Figure ASSAY: One unit of enzyme hydrolyzes 1microg of heat-denatured calf thymus DNAinto acid-soluble form at 37°C, pH4.6 in one minute. Composition of Supplied Reagents (Store at -20°C): 10xS1 Nuclease Buffer: 300mM Sodium acetate (pH4.6), 2800mM NaCl, 10mM ZnSO4

12352202