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S100 alpha9 Antibody (YA1894, Rabbit)

HY-P82149
MedChem Express
TargetS100a9
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    S100 alpha9 Antibody (YA1894, Rabbit)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID20202
  • Target name
    S100a9
  • Target description
    S100 calcium binding protein A9 (calgranulin B)
  • Target synonyms
    60B8Ag, BEE22, Cagb, GAGB, L1Ag, MRP14, p14, protein S100-A9, S100 calcium-binding protein A9, leukocyte L1 complex heavy chain, migration inhibitory factor-related protein 14
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    S100 alpha9 Antibody (YA1894) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA1894), targeting S100 alpha9, with a predicted molecular weight of 13 kDa (observed band size: 13 kDa). S100 alpha9 Antibody (YA1894) can be used for WB, IHC-F, IHC-P, ICC/IF experiment in mouse background.
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of S100A9 using anti-S100A9 antibody (PB9678). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse spleen tissue lysates, Lane 2: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-S100A9 antigen affinity purified polyclonal antibody (Catalog # PB9678) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for S100A9 at approximately 13-15 kDa. The expected band size for S100A9 is at 13 kDa.
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