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S100A9 Polyclonal Antibody

CAC10381
Biomatik
ApplicationsImmunoPrecipitation, Western Blot, ELISA
Product group Antibodies
ReactivityMouse
TargetS100a9
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Overview

  • Supplier
    Biomatik
  • Product Name
    S100A9 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    ImmunoPrecipitation, Western Blot, ELISA
  • Applications Supplier
    ELISA, WB, IP; Recommended dilution: WB:1:500-1:5000, IP:1:200-1:2000
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID20202
  • Target name
    S100a9
  • Target description
    S100 calcium binding protein A9 (calgranulin B)
  • Target synonyms
    60B8Ag, BEE22, Cagb, GAGB, L1Ag, MRP14, p14, protein S100-A9, S100 calcium-binding protein A9, leukocyte L1 complex heavy chain, migration inhibitory factor-related protein 14
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP31725
  • Protein Name
    Protein S100-A9
  • Scientific Description
    The S100A9 Polyclonal Antibody (Species: Mouse) has been validated for the following applications: ELISA, WB, IP.
  • Reactivity
    Mouse
  • Reactivity Supplier
    Mouse
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of S100A9 using anti-S100A9 antibody (PB9678). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse spleen tissue lysates, Lane 2: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-S100A9 antigen affinity purified polyclonal antibody (Catalog # PB9678) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for S100A9 at approximately 13-15 kDa. The expected band size for S100A9 is at 13 kDa.
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