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Western blot analysis of extracts of various cell lines using S100P Polyclonal Antibody at dilution of 1:1000.
Western blot analysis of extracts of various cell lines using S100P Polyclonal Antibody at dilution of 1:1000.
Western blot analysis of extracts of various cell lines using S100P Polyclonal Antibody at dilution of 1:1000.

S100P Polyclonal Antibody

E-AB-65108
Elabscience
Product group Antibodies
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Overview

  • Supplier
    Elabscience
  • Product Name
    S100P Polyclonal Antibody
  • Delivery Days Customer
    12
  • Certification
    Research Use Only
  • Scientific Description
    The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21; however, this gene is located at 4p16. This protein, in addition to binding Ca2+, also binds Zn2+ and Mg2+. This protein may play a role in the etiology of prostate cancer.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of S100P using anti-S100P antibody (A01963-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-S100P antigen affinity purified polyclonal antibody (Catalog # A01963-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for S100P at approximately 14 kDa. The expected band size for S100P is at 10 kDa.
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IHC-P analysis of human muscle-invasive bladder cancer (MIBC) tissue using GTX04398 S100P antibody [MSVA-480R] HistoMAX?. Muscle invasive urothelial carcinoma of the urinary bladder showing a strong S100P immunostaining of tumor cells.
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