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WB analysis of Jurkat lysate using GTX25645 SAE1 antibody, C-term. Dilution : 1microg/ml Loading : 30microg protein in RIPA buffer
WB analysis of Jurkat lysate using GTX25645 SAE1 antibody, C-term. Dilution : 1microg/ml Loading : 30microg protein in RIPA buffer
WB analysis of Jurkat lysate using GTX25645 SAE1 antibody, C-term. Dilution : 1microg/ml Loading : 30microg protein in RIPA buffer

SAE1 antibody, C-term

GTX25645
GeneTex
ApplicationsWestern Blot
Product group Antibodies
TargetSAE1
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Overview

  • Supplier
    GeneTex
  • Product Name
    SAE1 antibody, C-term
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1microg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.50 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID10055
  • Target name
    SAE1
  • Target description
    SUMO1 activating enzyme subunit 1
  • Target synonyms
    AOS1, HSPC140, SUA1, UBLE1A, SUMO-activating enzyme subunit 1, SUMO-1 activating enzyme E1 N subunit, SUMO-1 activating enzyme subunit 1, activator of SUMO1, sentrin/SUMO-activating protein AOS1, ubiquitin-like 1-activating enzyme E1A, ubiquitin-like protein SUMO-1 activating enzyme
  • Host
    Goat
  • Isotype
    IgG
  • Protein IDQ9UBE0
  • Protein Name
    SUMO-activating enzyme subunit 1
  • Scientific Description
    Posttranslational modification of proteins by the addition of the small protein SUMO (see SUMO1; MIM 601912), or sumoylation, regulates protein structure and intracellular localization. SAE1 and UBA2 (MIM 613295) form a heterodimer that functions as a SUMO-activating enzyme for the sumoylation of proteins (Okuma et al., 1999 [PubMed 9920803]).[supplied by OMIM, Mar 2010]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SAE1 using anti-SAE1 antibody (A04753-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: rat testis tissue lysates, Lane 6: mouse testis tissue lysates, Lane 7: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SAE1 antigen affinity purified polyclonal antibody (Catalog # A04753-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SAE1 at approximately 38 kDa. The expected band size for SAE1 is at 38 kDa.
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