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SAE1 Antibody (YA1847, Rabbit)

HY-P82102
MedChem Express
TargetSAE1
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    SAE1 Antibody (YA1847, Rabbit)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID10055
  • Target name
    SAE1
  • Target description
    SUMO1 activating enzyme subunit 1
  • Target synonyms
    AOS1, HSPC140, SUA1, UBLE1A, SUMO-activating enzyme subunit 1, SUMO-1 activating enzyme E1 N subunit, SUMO-1 activating enzyme subunit 1, activator of SUMO1, sentrin/SUMO-activating protein AOS1, ubiquitin-like 1-activating enzyme E1A, ubiquitin-like protein SUMO-1 activating enzyme
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    SAE1 Antibody (YA1847) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA1847), targeting SAE1, with a predicted molecular weight of 38 kDa (observed band size: 38 kDa). SAE1 Antibody (YA1847) can be used for WB, ICC/IF, IP, FC experiment in human, rat background.
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of SAE1 using anti-SAE1 antibody (A04753-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: rat testis tissue lysates, Lane 6: mouse testis tissue lysates, Lane 7: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SAE1 antigen affinity purified polyclonal antibody (Catalog # A04753-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SAE1 at approximately 38 kDa. The expected band size for SAE1 is at 38 kDa.
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