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The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using CSB-PA095524(SAMD9L Antibody) at dilution 1/30, on the right is treated with synthetic peptide. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using CSB-PA095524(SAMD9L Antibody) at dilution 1/30, on the right is treated with synthetic peptide. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using CSB-PA095524(SAMD9L Antibody) at dilution 1/30, on the right is treated with synthetic peptide. (Original magnification: x200)

SAMD9L Antibody

CSB-PA095524
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetSAMD9L
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Overview

  • Supplier
    Cusabio
  • Product Name
    SAMD9L Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID219285
  • Target name
    SAMD9L
  • Target description
    sterile alpha motif domain containing 9 like
  • Target synonyms
    ATXPC; C7orf6; DRIF2; M7MLS1; SAM domain-containing protein 9-like; sterile alpha motif domain-containing protein 9-like; UEF1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8IVG5
  • Protein Name
    Sterile alpha motif domain-containing protein 9-like
  • Scientific Description
    The deduced protein contains an N-terminal SAM domain. Database analysis indicated that SAMD9L can undergo alternative splicing leading to an alternative coding region. PCR analysis detected SAMD9L in all adult and fetal human tissues examined except some tumor tissues. Orthologs of SAMD9L were detected in all mammals examined but not in chicken, frog, or fish species. Widely expressed in adult and fetal tissues. Variable expression in tumors. Down-regulated in breast cancer.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of SAMD9L using anti-SAMD9L antibody (A12350-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U251 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SAMD9L antigen affinity purified polyclonal antibody (Catalog # A12350-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SAMD9L at approximately 200 kDa. The expected band size for SAMD9L is at 185 kDa.
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