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ProductsBack/SAMHD1 antibody [1F6]
WB analysis of HEK293T cells transfected with SAMHD1 plasmid (Right) or empty vector (Left) for 48 hrs using GTX83687 SAMHD1 antibody [1F6]. Loading : 5 ug per lane
WB analysis of HEK293T cells transfected with SAMHD1 plasmid (Right) or empty vector (Left) for 48 hrs using GTX83687 SAMHD1 antibody [1F6]. Loading : 5 ug per lane
WB analysis of HEK293T cells transfected with SAMHD1 plasmid (Right) or empty vector (Left) for 48 hrs using GTX83687 SAMHD1 antibody [1F6]. Loading : 5 ug per lane

SAMHD1 antibody [1F6]

GTX83687
GeneTex
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman, Monkey, Mouse
TargetSAMHD1
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Overview

  • Supplier
    GeneTex
  • Product Name
    SAMHD1 antibody [1F6]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:2000. ICC/IF: 1:100. FCM: 1:100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    1F6
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID25939
  • Target name
    SAMHD1
  • Target description
    SAM and HD domain containing deoxynucleoside triphosphate triphosphohydrolase 1
  • Target synonyms
    CHBL2, DCIP, HDDC1, MOP-5, SBBI88, hSAMHD1, deoxynucleoside triphosphate triphosphohydrolase SAMHD1, SAM domain and HD domain 1, SAM domain and HD domain-containing protein 1, dNTPase, dendritic cell-derived IFNG-induced protein, monocyte protein 5
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ9Y3Z3
  • Protein Name
    Deoxynucleoside triphosphate triphosphohydrolase SAMHD1
  • Scientific Description
    Putative nuclease involved in innate immune response by acting as a negative regulator of the cell-intrinsic antiviral response. May play a role in mediating proinflammatory responses to TNF-alpha signaling.
  • Reactivity
    Human, Monkey, Mouse
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    41116161

References

  • Positive selection in dNTPase SAMHD1 throughout mammalian evolution. Monit C et al., 2019 Sep 10, Proc Natl Acad Sci U S A
    Read this paper
  • Sulforaphane Inhibits HIV Infection of Macrophages through Nrf2. Furuya AK et al., 2016 Apr, PLoS Pathog
    Read this paper
  • Cullin4A and cullin4B are interchangeable for HIV Vpr and Vpx action through the CRL4 ubiquitin ligase complex. Sharifi HJ et al., 2014 Jun, J Virol
    Read this paper
  • HIV relies on neddylation for ubiquitin ligase-mediated functions. Nekorchuk MD et al., 2013 Nov 18, Retrovirology
    Read this paper

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Figure 1. IF analysis of SAMHD1 using anti-SAMHD1 antibody (A00592-4). SAMHD1 was detected in immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5microg/mL rabbit anti-SAMHD1 Antibody (A00592-4) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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