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ProductsBack/SAP30 Antibody (aa170-220)

SAP30 Antibody (aa170-220)

LS-C290217
LifeSpan BioSciences
ApplicationsImmunoPrecipitation, Western Blot
Product group Antibodies
TargetSAP30
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    SAP30 Antibody (aa170-220)
  • Delivery Days Customer
    23
  • Applications
    ImmunoPrecipitation, Western Blot
  • Applications Supplier
    IP (2 - 10 µg/mg lysate), WB (1:1000 - 1:5000)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID8819
  • Target name
    SAP30
  • Target description
    Sin3A associated protein 30
  • Target synonyms
    histone deacetylase complex subunit SAP30, 30 kDa Sin3-associated polypeptide, Sin3 corepressor complex subunit SAP30, Sin3-associated polypeptide, 30kDa, Sin3A-associated protein, 30kDa, sin3-associated polypeptide p30, sin3-associated polypeptide, 30 kDa
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SAP30 using anti-SAP30 antibody (A06526-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SAP30 antigen affinity purified polyclonal antibody (Catalog # A06526-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SAP30 at approximately 28 kDa. The expected band size for SAP30 is at 28 kDa.
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