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SAPS3 Polyclonal Antibody

BS-19390R
Bioss
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetPPP6R3
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Overview

  • Supplier
    Bioss
  • Product Name
    SAPS3 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID55291
  • Target name
    PPP6R3
  • Target description
    protein phosphatase 6 regulatory subunit 3
  • Target synonyms
    C11orf23, PP6R3, SAP190, SAPL, SAPLa, SAPS3, serine/threonine-protein phosphatase 6 regulatory subunit 3, SAPS domain family, member 3, sporulation-induced transcript 4-associated protein SAPL
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ5H9R7
  • Protein Name
    Serine/threonine-protein phosphatase 6 regulatory subunit 3
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SAPS3/PPP6R3 using anti-SAPS3/PPP6R3 antibody (A10833-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human U-87MG whole cell lysates, Lane 4: monkey COS-7 whole cell lysates, Lane 5: rat NRK whole cell lysates, Lane 6: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SAPS3/PPP6R3 antigen affinity purified polyclonal antibody (Catalog # A10833-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SAPS3/PPP6R3 at approximately 144 kDa. The expected band size for SAPS3/PPP6R3 is at 95 kDa.
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