
SARS-CoV-2 nsp12 detects SARS-CoV-2 nsp12 protein at cytoplasm by immunohistochemical analysis. Sample: human SARS-CoV-2 (COVID-19) Nucleocapsid transfected 293T cell FFPE Cell Pellet Block (GTX435641). Green: SARS-CoV-2 nsp12 stained by SARS-CoV-2 nsp12 (GTX636916) diluted at 1:255. Blue: Fluoroshield with DAPI (GTX30920). Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
SARS-CoV-2 (COVID-19) nsp12 / RdRP antibody [HL1444]
GTX636916
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityVirus
Overview
- SupplierGeneTex
- Product NameSARS-CoV-2 (COVID-19) nsp12 / RdRP antibody [HL1444]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:1000-1:10000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHL1444
- Concentration1 mg/ml
- ConjugateUnconjugated
- HostRabbit
- IsotypeIgG
- ReactivityVirus
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
![SARS-CoV-2 (COVID-19) nsp12 / RdRP antibody [HL1444] detects SARS-CoV-2 (COVID-19) nsp12 / RdRP protein at cytoplasm by immunofluorescent analysis. Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SARS-CoV-2 (COVID-19) nsp12 / RdRP stained by SARS-CoV-2 (COVID-19) nsp12 / RdRP antibody [HL1444] (GTX636916) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920). Scale bar= 10μm. SARS-CoV-2 (COVID-19) nsp12 / RdRP antibody [HL1444] detects SARS-CoV-2 (COVID-19) nsp12 / RdRP protein at cytoplasm by immunofluorescent analysis. Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SARS-CoV-2 (COVID-19) nsp12 / RdRP stained by SARS-CoV-2 (COVID-19) nsp12 / RdRP antibody [HL1444] (GTX636916) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920). Scale bar= 10μm.](https://www.genetex.com/upload/website/prouct_img/normal/GTX636916/GTX636916_T-44641_20220429_ICC_IF_B_SARSCoronavirus2_w_23061202_325.webp)
![Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nsp12 / RdRP antibody [HL1444] (GTX636916) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nsp12 / RdRP antibody [HL1444] (GTX636916) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX636916/GTX636916_45278_20240105_WB_SARSCoronavirus2_B_25021320_728.webp)