Indirect ELISA analysis was performed by coating plate with 50 μL of recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein (GTX135357-pro) at concentrations ranging from 0.0625 μg/mL to 4 μg/mL. The coated protein is detected with SARS-CoV-2 (COVID-19) nucleocapsid antibody (GTX135357) at 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.
![Sandwich ELISA detection of non-transfected and SARS-CoV-2 nucleocapsid (full length) transfected 293T whole cell extracts using SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] (GTX632269) as capture antibody at concentration of 5 弮g/mL and SARS-CoV-2 (COVID-19) nucleocapsid antibody (GTX135357) as detection antibody at concentration of 1 弮g/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX135357/GTX135357_43941_20200605_ELISA_PAIR_B_w_23060620_390.webp "Sandwich ELISA detection of non-transfected and SARS-CoV-2 nucleocapsid (full length) transfected 293T whole cell extracts using SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] (GTX632269) as capture antibody at concentration of 5 弮g/mL and SARS-CoV-2 (COVID-19) nucleocapsid antibody (GTX135357) as detection antibody at concentration of 1 弮g/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.")
![SARS-CoV-2 (COVID-19) nucleocapsid antibody detects SARS-CoV-2 (COVID-19) nucleocapsid protein by immunohistochemical analysis. Sample: Paraffin-embedded SARS-CoV-2 (COVID-19) Nucleocapsid FFPE Cell Pellet Block. Green: SARS-CoV-2 (COVID-19) nucleocapsid stained by SARS-CoV-2 (COVID-19) nucleocapsid antibody (GTX135357) diluted at 1:1000. Red: SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid stained by SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] (GTX632269) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920). Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX135357/GTX135357_43931_20200430_IHC-P-FL_Virus_1_w_23060620_286.webp "SARS-CoV-2 (COVID-19) nucleocapsid antibody detects SARS-CoV-2 (COVID-19) nucleocapsid protein by immunohistochemical analysis. Sample: Paraffin-embedded SARS-CoV-2 (COVID-19) Nucleocapsid FFPE Cell Pellet Block. Green: SARS-CoV-2 (COVID-19) nucleocapsid stained by SARS-CoV-2 (COVID-19) nucleocapsid antibody (GTX135357) diluted at 1:1000. Red: SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid stained by SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] (GTX632269) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920). Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
Indirect ELISA analysis was performed by coating plate with 50 μL of recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein (GTX135357-pro) at concentrations ranging from 0.0625 μg/mL to 4 μg/mL. The coated protein is detected with SARS-CoV-2 (COVID-19) nucleocapsid antibody (GTX135357) at 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.
SARS-CoV-2 (COVID-19) Nucleocapsid antibody
GTX135357
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin, Neutralisation/Blocking
Product group Antibodies
ReactivityVirus
Overview
- SupplierGeneTex
- Product NameSARS-CoV-2 (COVID-19) Nucleocapsid antibody
- Delivery Days Customer9
- Application Supplier NoteWB: 1:1000-1:10000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:5000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin, Neutralisation/Blocking
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration0.33 mg/ml
- ConjugateUnconjugated
- HostRabbit
- IsotypeIgG
- ReactivityVirus
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161