Sandwich ELISA detection of recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag protein (GTX135592-pro) using SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL146] (GTX635680) as capture antibody at concentration of 5 μg/mL and HRP-conjugated SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL249] (GTX635678) as detection antibody at concentration of 1 μg/mL.
![Sandwich ELISA detection of recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag protein (GTX135592-pro) using SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL146] (GTX635680) as capture antibody at concentration of 5 μg/mL and HRP-conjugated SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL455] (GTX635688) as detection antibody at concentration of 1 μg/mL.](https://www.genetex.com/upload/website/prouct_img/normal/GTX635680/GTX635680_43978_20200610_ELISA_PAIR_2_w_23061202_278.webp "Sandwich ELISA detection of recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag protein (GTX135592-pro) using SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL146] (GTX635680) as capture antibody at concentration of 5 μg/mL and HRP-conjugated SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL455] (GTX635688) as detection antibody at concentration of 1 μg/mL.")
![SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL146] detects SARS-CoV-2 (COVID-19) nucleocapsid protein by immunofluorescent analysis. Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SARS-CoV-2 (COVID-19) nucleocapsid stained by SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL146] (GTX635680) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920).](https://www.genetex.com/upload/website/prouct_img/normal/GTX635680/GTX635680_43964_20201203_ICC_IF_SARSCoronavirus2_B_w_23061202_740.webp "SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL146] detects SARS-CoV-2 (COVID-19) nucleocapsid protein by immunofluorescent analysis. Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SARS-CoV-2 (COVID-19) nucleocapsid stained by SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL146] (GTX635680) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920).")
![Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL146] (GTX635680) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX635680/GTX635680_43992_20200703_WB_SARScoronavirus2_B_w_23061202_997.webp "Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL146] (GTX635680) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Indirect ELISA analysis performed by coating plate with recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein (GTX135592-pro) (50 ng). Coated protein probed with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL146] (GTX635680) (2.4*10?? ~ 20 nM). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) detected bound primary antibody. EC50 : 0.05 nM](https://www.genetex.com/upload/website/prouct_img/normal/GTX635680/GTX635680_43964_20200529_ELISA_Indirect_w_23061202_723.webp "Indirect ELISA analysis performed by coating plate with recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein (GTX135592-pro) (50 ng). Coated protein probed with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL146] (GTX635680) (2.4*10?? ~ 20 nM). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) detected bound primary antibody. EC50 : 0.05 nM")
![Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL146] (GTX635680) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX635680/GTX635680_43964_20200526_WB_B_multiplevirus_w_23061202_435.webp "Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL146] (GTX635680) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
Sandwich ELISA detection of recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag protein (GTX135592-pro) using SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL146] (GTX635680) as capture antibody at concentration of 5 μg/mL and HRP-conjugated SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL249] (GTX635678) as detection antibody at concentration of 1 μg/mL.
SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL146]
GTX635680
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
Product group Antibodies
ReactivityVirus
Overview
- SupplierGeneTex
- Product NameSARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL146]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:1000-1:10000. ICC/IF: 1:100-1:1000. Sandwich ELISA: . *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHL146
- Concentration1 mg/ml
- ConjugateUnconjugated
- HostRabbit
- IsotypeIgG
- ReactivityVirus
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161