Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL453] (GTX635687) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
![SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL453] detects SARS-CoV-2 (COVID-19) nucleocapsid protein by immunofluorescent analysis. Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SARS-CoV-2 (COVID-19) nucleocapsid stained by SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL453] (GTX635687) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920).](https://www.genetex.com/upload/website/prouct_img/normal/GTX635687/GTX635687_43978_20200528_ICC_IF_SARSCoronavirus2_B_w_23061202_365.webp "SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL453] detects SARS-CoV-2 (COVID-19) nucleocapsid protein by immunofluorescent analysis. Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SARS-CoV-2 (COVID-19) nucleocapsid stained by SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL453] (GTX635687) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920).")
![SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL453] detects SARS-CoV-2 (COVID-19) nucleocapsid protein by immunohistochemical analysis. Sample: Mock (GTX435670) and SARS-CoV-2 (COVID-19) Nucleocapsid transfected 293T cell FFPE Cell Pellet Block (GTX435641). Green: SARS-CoV-2 (COVID-19) nucleocapsid stained by SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL453] (GTX635687) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920). Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX635687/GTX635687_43999_20200724_IHC-P-FL_B_w_23061202_334.webp "SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL453] detects SARS-CoV-2 (COVID-19) nucleocapsid protein by immunohistochemical analysis. Sample: Mock (GTX435670) and SARS-CoV-2 (COVID-19) Nucleocapsid transfected 293T cell FFPE Cell Pellet Block (GTX435641). Green: SARS-CoV-2 (COVID-19) nucleocapsid stained by SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL453] (GTX635687) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920). Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![SARS-CoV-2 (COVID-19) nucleocapsid protein, B.1.1.529 / Omicron variant, His tag (0.5 μg), expressed by HEK293 cells (GTX136779-pro) or E. coli (GTX03400-pro), were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL453] (GTX635687) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX635687/GTX635687_44048_20220311_WB_SARSCoronavirus2_Ag_w_23061202_897.webp "SARS-CoV-2 (COVID-19) nucleocapsid protein, B.1.1.529 / Omicron variant, His tag (0.5 μg), expressed by HEK293 cells (GTX136779-pro) or E. coli (GTX03400-pro), were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL453] (GTX635687) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Indirect ELISA analysis performed by coating plate with recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein (GTX135592-pro) (50 ng). Coated protein probed with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL453] (GTX635687) (4.8*10?? ~ 20 nM). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) detected bound primary antibody. EC50 : 0.006 nM](https://www.genetex.com/upload/website/prouct_img/normal/GTX635687/GTX635687_43978_20200529_ELISA_Indirect_w_23061202_600.webp "Indirect ELISA analysis performed by coating plate with recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein (GTX135592-pro) (50 ng). Coated protein probed with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL453] (GTX635687) (4.8*10?? ~ 20 nM). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) detected bound primary antibody. EC50 : 0.006 nM")
![Indirect ELISA analysis performed by coating plate with recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein (GTX135592-pro) (15.63-1000 ng/mL). Coated protein probed with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL453] (GTX635687) (1 μg/mL). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) detected bound primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX635687/GTX635687_43978_20200529_ELISA_Indirect_2_w_23061202_432.webp "Indirect ELISA analysis performed by coating plate with recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein (GTX135592-pro) (15.63-1000 ng/mL). Coated protein probed with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL453] (GTX635687) (1 μg/mL). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) detected bound primary antibody.")
![Non-transfected (–) and transfected (+) Vero E6 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL453] (GTX635687) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX635687/GTX635687_43999_20201009_WB_SARScoronavirus2_B_w_23061202_429.webp "Non-transfected (–) and transfected (+) Vero E6 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL453] (GTX635687) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL453] (GTX635687) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](https://www.genetex.com/upload/website/prouct_img/normal/GTX635687/GTX635687_43978_20200612_WB_multiplevirus_B_w_23061202_195.webp "Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL453] (GTX635687) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL453] (GTX635687) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL453]
GTX635687
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityVirus
Overview
- SupplierGeneTex
- Product NameSARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL453]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:1000-1:10000. ICC/IF: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHL453
- Concentration1 mg/ml
- ConjugateUnconjugated
- HostRabbit
- IsotypeIgG
- ReactivityVirus
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161