Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5410] (GTX635685) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
![Sandwich ELISA detection of recombinant nucleocapsid protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; BA.2 / BA.5 Omicron variant) using antibodies as below. Capture: SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5410] (GTX635685) (5 μg/mL) Detection: HRP-conjugated SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL455] (GTX635688) (1 μg/mL) Please notice that GTX635688 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635685. Please contact us for custom HRP-conjugated antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX635685/GTX635685_44606_20220729_ELISA_PAIR_Multiple_22080119_740.webp "Sandwich ELISA detection of recombinant nucleocapsid protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; BA.2 / BA.5 Omicron variant) using antibodies as below. Capture: SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5410] (GTX635685) (5 μg/mL) Detection: HRP-conjugated SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL455] (GTX635688) (1 μg/mL) Please notice that GTX635688 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635685. Please contact us for custom HRP-conjugated antibody.")
![Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Nucleocapsid protein, Omicron / BA.4 variant, His tag using antibodies as below. Capture: SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5410] (GTX635685) (5 μg/mL) Detection: HRP-conjugated SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL455] (GTX635688) (1 μg/mL) Please notice that GTX635688 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635685. Please contact us for custom HRP-conjugated antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX635685/GTX635685_44606_20221007_ELISA_Sandwich_22101319_733.webp "Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Nucleocapsid protein, Omicron / BA.4 variant, His tag using antibodies as below. Capture: SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5410] (GTX635685) (5 μg/mL) Detection: HRP-conjugated SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL455] (GTX635688) (1 μg/mL) Please notice that GTX635688 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635685. Please contact us for custom HRP-conjugated antibody.")
![Indirect ELISA analysis performed by coating plate with recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag protein (GTX135592-pro) (50 ng). Coated protein probed with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5410] (GTX635685) (20-4.8*10 -6 nM). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) detected bound primary antibody. EC50 : 0.009 nM](https://www.genetex.com/upload/website/prouct_img/normal/GTX635685/GTX635685_43978_20200807_ELISA_Indirect_EC50_w_23061202_486.webp "Indirect ELISA analysis performed by coating plate with recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag protein (GTX135592-pro) (50 ng). Coated protein probed with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5410] (GTX635685) (20-4.8*10 -6 nM). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) detected bound primary antibody. EC50 : 0.009 nM")
![SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5410] detects SARS-CoV-2 (COVID-19) nucleocapsid protein by immunohistochemical analysis. Sample: Mock (GTX435670) and SARS-CoV-2 (COVID-19) Nucleocapsid transfected 293T cell FFPE Cell Pellet Block (GTX435641). Green: SARS-CoV-2 (COVID-19) nucleocapsid stained by SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5410] (GTX635685) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920). Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX635685/GTX635685_43999_20200724_IHC-P-FL_B_w_23061202_600.webp "SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5410] detects SARS-CoV-2 (COVID-19) nucleocapsid protein by immunohistochemical analysis. Sample: Mock (GTX435670) and SARS-CoV-2 (COVID-19) Nucleocapsid transfected 293T cell FFPE Cell Pellet Block (GTX435641). Green: SARS-CoV-2 (COVID-19) nucleocapsid stained by SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5410] (GTX635685) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920). Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Sandwich ELISA detection of non-transfected and SARS-CoV-2, MERS-CoV and SARS-CoV Nucleocapsid transfected 293T whole cell extracts, SARS-CoV OC43 lysate, SARS-CoV NL63 lysate, or SARS-CoV 229E lysate using SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5410] (GTX635685) as capture antibody at concentration of 5 弮g/mL and SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL455-MS] (GTX635712) as detection antibody at concentration of 1 弮g/mL. Mouse IgG antibody (HRP) (GTX213111-01) was diluted at 1:10000 and used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX635685/GTX635685_44048_20210104_ELISA_PAIR_multiplevirus_B_w_23061202_587.webp "Sandwich ELISA detection of non-transfected and SARS-CoV-2, MERS-CoV and SARS-CoV Nucleocapsid transfected 293T whole cell extracts, SARS-CoV OC43 lysate, SARS-CoV NL63 lysate, or SARS-CoV 229E lysate using SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5410] (GTX635685) as capture antibody at concentration of 5 弮g/mL and SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL455-MS] (GTX635712) as detection antibody at concentration of 1 弮g/mL. Mouse IgG antibody (HRP) (GTX213111-01) was diluted at 1:10000 and used to detect the primary antibody.")
![Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag protein (GTX135592-pro or GTX135357-pro) (15.63-1000 ng/mL). Coated protein probed with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5410] (GTX635685) (1 μg/mL). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) detected bound primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX635685/GTX635685_43979_20200612_ELISA_Indirect_w_23061202_736.webp "Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag protein (GTX135592-pro or GTX135357-pro) (15.63-1000 ng/mL). Coated protein probed with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5410] (GTX635685) (1 μg/mL). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) detected bound primary antibody.")
![SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5410] detects SARS-CoV-2 (COVID-19) nucleocapsid protein by immunofluorescent analysis. Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SARS-CoV-2 (COVID-19) nucleocapsid stained by SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5410] (GTX635685) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920).](https://www.genetex.com/upload/website/prouct_img/normal/GTX635685/GTX635685_43979_20200610_ICC_IF_SARScoronavirus2_B_w_23061202_829.webp "SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5410] detects SARS-CoV-2 (COVID-19) nucleocapsid protein by immunofluorescent analysis. Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SARS-CoV-2 (COVID-19) nucleocapsid stained by SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5410] (GTX635685) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920).")
![Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5410] (GTX635685) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX635685/GTX635685_43979_20200612_WB_multiplevirus_B_w_23061202_391.webp "Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5410] (GTX635685) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Sandwich ELISA detection of recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag protein (GTX135592-pro) using SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5410] (GTX635685) as capture antibody at concentration of 5 μg/mL and SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL455-MS] (GTX635712) as detection antibody at concentration of 1 μg/mL. Mouse IgG antibody (HRP) (GTX213111-01) was diluted at 1:10000 and used to detect the primary antibody. Sensitivity : 2.68 pg/mL](https://www.genetex.com/upload/website/prouct_img/normal/GTX635685/GTX635685_43979_20200821_ELISA_PAIR_w_23061202_150.webp "Sandwich ELISA detection of recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag protein (GTX135592-pro) using SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5410] (GTX635685) as capture antibody at concentration of 5 μg/mL and SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL455-MS] (GTX635712) as detection antibody at concentration of 1 μg/mL. Mouse IgG antibody (HRP) (GTX213111-01) was diluted at 1:10000 and used to detect the primary antibody. Sensitivity : 2.68 pg/mL")
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5410] (GTX635685) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL5410]
GTX635685
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, Other Application
Product group Antibodies
ReactivityVirus
Overview
- SupplierGeneTex
- Product NameSARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL5410]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:1000-1:10000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, Other Application
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHL5410
- Concentration1 mg/ml
- ConjugateUnconjugated
- HostRabbit
- IsotypeIgG
- ReactivityVirus
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161