Sandwich ELISA detection of recombinant nucleocapsid protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; BA.2 / BA.5 Omicron variant) using antibodies as below. Capture: SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] (GTX635689) (5 μg/mL) Detection: SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] (HRP) (GTX635686-01) (1 μg/mL)
![Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Nucleocapsid protein, Omicron / BA.2 / BA.5 variant, His tag (GTX137219-pro) using antibodies as below. Capture: SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL455] (GTX635688) (5 μg/mL) Detection: HRP-conjugated SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] (GTX635689) (1 μg/mL) Please notice that GTX635689 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635688. Please contact us for custom HRP-conjugated antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX635689/GTX635689_44676_20220916_ELISA_PAIR_22092119_544.webp "Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Nucleocapsid protein, Omicron / BA.2 / BA.5 variant, His tag (GTX137219-pro) using antibodies as below. Capture: SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL455] (GTX635688) (5 μg/mL) Detection: HRP-conjugated SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] (GTX635689) (1 μg/mL) Please notice that GTX635689 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635688. Please contact us for custom HRP-conjugated antibody.")
![Indirect ELISA analysis was performed by coating the plate with recombinant SARS-CoV-2 (COVID-19) Nucleocapsid protein, Omicron / BA.2 / BA.5 variant, His tag (GTX137219-pro) (21.23-0.31 nM). Coated protein was probed with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] (GTX635689) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX635689/GTX635689_44683_20220916_ELISA_Indirect_22092119_206.webp "Indirect ELISA analysis was performed by coating the plate with recombinant SARS-CoV-2 (COVID-19) Nucleocapsid protein, Omicron / BA.2 / BA.5 variant, His tag (GTX137219-pro) (21.23-0.31 nM). Coated protein was probed with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] (GTX635689) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.")
![Sandwich ELISA detection of recombinant nucleocapsid protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; BF.7 Omicron variant; BQ.1 Omicron variant) using antibodies as below. Capture: SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] (GTX635689) (5 μg/mL) Detection: HRP-conjugated SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] (GTX635686) (1 μg/mL) Please notice that GTX635686 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635689. Please contact us for custom HRP-conjugated antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX635689/GTX635689_44676_20221230_ELISA_PAIR_Multiple_23010400_781.webp "Sandwich ELISA detection of recombinant nucleocapsid protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; BF.7 Omicron variant; BQ.1 Omicron variant) using antibodies as below. Capture: SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] (GTX635689) (5 μg/mL) Detection: HRP-conjugated SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] (GTX635686) (1 μg/mL) Please notice that GTX635686 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635689. Please contact us for custom HRP-conjugated antibody.")
![Sandwich ELISA detection of recombinant nucleocapsid protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; BF.7 Omicron variant; BQ.1 Omicron variant) using antibodies as below. Capture: SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL455] (GTX635688) (5 μg/mL) Detection: HRP-conjugated SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] (GTX635689) (1 μg/mL) Please notice that GTX635689 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635688. Please contact us for custom HRP-conjugated antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX635689/GTX635689_T-44788_20221230_ELISA_PAIR_Multiple_23010400_293.webp "Sandwich ELISA detection of recombinant nucleocapsid protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; BF.7 Omicron variant; BQ.1 Omicron variant) using antibodies as below. Capture: SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL455] (GTX635688) (5 μg/mL) Detection: HRP-conjugated SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] (GTX635689) (1 μg/mL) Please notice that GTX635689 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635688. Please contact us for custom HRP-conjugated antibody.")
![Sandwich ELISA detection of recombinant full-length Nucleocapsid proteins derived from different strains of SARS-CoV-2 (COVID-19) (Wild type or B.1.617.2 delta variant) using antibodies as below.
Capture: SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] (GTX635689) (5 μg/mL)
Detection: HRP-conjugated SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] (GTX635686) (1 μg/mL).
Please notice that GTX635686 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635689. Please contact us for custom HRP-conjugated antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX635689/GTX635689_44235_20210723_ELISA_PAIR_Multiple_w_23061202_697.webp "Sandwich ELISA detection of recombinant full-length Nucleocapsid proteins derived from different strains of SARS-CoV-2 (COVID-19) (Wild type or B.1.617.2 delta variant) using antibodies as below.
Capture: SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] (GTX635689) (5 μg/mL)
Detection: HRP-conjugated SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] (GTX635686) (1 μg/mL).
Please notice that GTX635686 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635689. Please contact us for custom HRP-conjugated antibody.")
![Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag (GTX135592-pro) using antibodies as below. Capture: SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] (GTX635689) (5 μg/mL) Detection: SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] (HRP) (GTX635686-01) (1 μg/mL)](https://www.genetex.com/upload/website/prouct_img/normal/GTX635689/GTX635689_44606_20220304_ELISA_PAIR_w_23061202_686.webp "Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag (GTX135592-pro) using antibodies as below. Capture: SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] (GTX635689) (5 μg/mL) Detection: SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] (HRP) (GTX635686-01) (1 μg/mL)")
![SARS-CoV-2 (COVID-19) nucleocapsid protein, B.1.1.529 / omicron variant, His tag (1 μg, GTX136779-pro) were separated by 12% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] (GTX635689) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX635689/GTX635689_44522_20220125_WB_SARScoronavirus2_Ag_w_23061202_130.webp "SARS-CoV-2 (COVID-19) nucleocapsid protein, B.1.1.529 / omicron variant, His tag (1 μg, GTX136779-pro) were separated by 12% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] (GTX635689) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![SARS-CoV-2 (COVID-19) nucleocapsid protein, B.1.1.529 / Omicron variant, His tag (0.5 μg), expressed by HEK293 cells (GTX136779-pro) or E. coli (GTX03400-pro), were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] (GTX635689) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX635689/GTX635689_44235_20220311_WB_SARSCoronavirus2_Ag_w_23061202_457.webp "SARS-CoV-2 (COVID-19) nucleocapsid protein, B.1.1.529 / Omicron variant, His tag (0.5 μg), expressed by HEK293 cells (GTX136779-pro) or E. coli (GTX03400-pro), were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] (GTX635689) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] detects SARS-CoV-2 (COVID-19) nucleocapsid protein by immunofluorescent analysis. Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SARS-CoV-2 (COVID-19) nucleocapsid stained by SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] (GTX635689) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920).](https://www.genetex.com/upload/website/prouct_img/normal/GTX635689/GTX635689_43978_20200528_ICC_IF_SARSCoronavirus2_B_w_23061202_411.webp "SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] detects SARS-CoV-2 (COVID-19) nucleocapsid protein by immunofluorescent analysis. Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SARS-CoV-2 (COVID-19) nucleocapsid stained by SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] (GTX635689) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920).")
Sandwich ELISA detection of recombinant nucleocapsid protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; BA.2 / BA.5 Omicron variant) using antibodies as below. Capture: SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] (GTX635689) (5 μg/mL) Detection: SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] (HRP) (GTX635686-01) (1 μg/mL)
SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL5511]
GTX635689
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin, Other Application
Product group Antibodies
ReactivityVirus
Overview
- SupplierGeneTex
- Product NameSARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL5511]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:1000-1:10000. ICC/IF: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin, Other Application
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHL5511
- Concentration1 mg/ml
- ConjugateUnconjugated
- HostRabbit
- IsotypeIgG
- ReactivityVirus
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Kong W, Montano M, Corley MJ, et al. Neuropilin-1 Mediates SARS-CoV-2 Infection of Astrocytes in Brain Organoids, Inducing Inflammation Leading to Dysfunction and Death of Neurons. mBio. 2022,13(6):e0230822. doi: 10.1128/mbio.02308-22Read this paper
- Yoo JS, Sasaki M, Cho SX, et al. SARS-CoV-2 inhibits induction of the MHC class I pathway by targeting the STAT1-IRF1-NLRC5 axis. Nat Commun. 2021,12(1):6602. doi: 10.1038/s41467-021-26910-8Read this paper
- Lee CY, Huang CH, Rastegari E, et al. Tumor Necrosis Factor-Alpha Exacerbates Viral Entry in SARS-CoV2-Infected iPSC-Derived Cardiomyocytes. Int J Mol Sci. 2021,22(18). doi: 10.3390/ijms22189869Read this paper
- Qu Y, Wang X, Zhu Y, et al. ORF3a-Mediated Incomplete Autophagy Facilitates Severe Acute Respiratory Syndrome Coronavirus-2 Replication. Front Cell Dev Biol. 2021,9:716208. doi: 10.3389/fcell.2021.716208Read this paper
- Plescia CB, David EA, Patra D, et al. SARS-CoV-2 viral budding and entry can be modeled using BSL-2 level virus-like particles. J Biol Chem. 2021,296:100103. doi: 10.1074/jbc.RA120.016148Read this paper