Functional ELISA analysis of immobilized recombinant Spike ECD protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; BA.1 Omicron variant; BA.2 Omicron variant; BA.4/BA.5 Omicron variant) (coated at 2 μg/mL) binding to soluble recombinant Human ACE2 (ECD) protein, mouse IgG Fc tag (active) (GTX135683-pro) (0.16-117.19 nM). Bound protein was detected by Goat Anti-Mouse IgG antibody (HRP) (GTX213111-01) (1:10000).
![Sandwich ELISA detection of recombinant Spike ECD protein(s) derived from different strains of SARS-CoV-2 virus (ie., BA.1 Omicron variant; BA.2 Omicron variant; BA.4/BA.5 Omicron variant) using antibodies as below. Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) (5 μg/mL) Detection: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1004] (HRP) (GTX635793-01) (1 μg/mL)](https://www.genetex.com/upload/website/prouct_img/normal/GTX136780-pro/GTX136780-pro_44638_20220715_ELISA_PAIR_Multiple_22071823_655.webp "Sandwich ELISA detection of recombinant Spike ECD protein(s) derived from different strains of SARS-CoV-2 virus (ie., BA.1 Omicron variant; BA.2 Omicron variant; BA.4/BA.5 Omicron variant) using antibodies as below. Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) (5 μg/mL) Detection: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1004] (HRP) (GTX635793-01) (1 μg/mL)")
![Sandwich ELISA detection of recombinant Spike ECD protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; B.1.1.529 Omicron variant) using antibodies as below. Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) (5 μg/mL) Detection: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] (HRP) (GTX635792-01) (1 μg/mL)](https://www.genetex.com/upload/website/prouct_img/normal/GTX136780-pro/GTX136780-pro_44589_20220211_ELISA_PAIR_Multiple_w_23060620_428.webp "Sandwich ELISA detection of recombinant Spike ECD protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; B.1.1.529 Omicron variant) using antibodies as below. Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) (5 μg/mL) Detection: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] (HRP) (GTX635792-01) (1 μg/mL)")
![Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) Spike (ECD) Protein, B.1.1.529 / Omicron variant, His tag (GTX136780-pro) (29.71-0.46 nM). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807), SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] (GTX635792), and SARS-CoV-2 (COVID-19) Spike S2 antibody [HL237] (GTX635693) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX136780-pro/GTX136780-pro_44589_20220211_ELISA_Indirect_Multiple_w_23060620_239.webp "Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) Spike (ECD) Protein, B.1.1.529 / Omicron variant, His tag (GTX136780-pro) (29.71-0.46 nM). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807), SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] (GTX635792), and SARS-CoV-2 (COVID-19) Spike S2 antibody [HL237] (GTX635693) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.")
![SARS-CoV-2 (COVID-19) Spike (ECD) Protein, B.1.1.529 / omicron variant, His tag (1 μg, GTX136780-pro) were separated by 12% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] (GTX635692) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX136780-pro/GTX136780-pro_44589_20220127_WB_SARScoronavirus2_Ag_w_23060620_493.webp "SARS-CoV-2 (COVID-19) Spike (ECD) Protein, B.1.1.529 / omicron variant, His tag (1 μg, GTX136780-pro) were separated by 12% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] (GTX635692) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) Spike (ECD) Protein, B.1.1.529 / Omicron variant, His tag (GTX136780-pro) (29.71-0.46 nM). Coated protein was probed with SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) (1 μg/mL). Goat anti-mouse IgG antibody (HRP) (GTX213111-01) (1:10000) was used to detect bound primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX136780-pro/GTX136780-pro_44589_20220211_ELISA_Indirect_w_23060620_822.webp "Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) Spike (ECD) Protein, B.1.1.529 / Omicron variant, His tag (GTX136780-pro) (29.71-0.46 nM). Coated protein was probed with SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) (1 μg/mL). Goat anti-mouse IgG antibody (HRP) (GTX213111-01) (1:10000) was used to detect bound primary antibody.")
![SARS-CoV-2 (COVID-19) Spike (ECD) Protein, B.1.1.529 / Omicron variant, His tag (1 μg, GTX136780-pro) were separated by 12% SDS-PAGE, and the membrane was blotted with SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX136780-pro/GTX136780-pro_44589_20220127_WB_SARScoronavirus2_Ag_2_w_23060620_349.webp "SARS-CoV-2 (COVID-19) Spike (ECD) Protein, B.1.1.529 / Omicron variant, His tag (1 μg, GTX136780-pro) were separated by 12% SDS-PAGE, and the membrane was blotted with SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.")
Functional ELISA analysis of immobilized recombinant Spike ECD protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; BA.1 Omicron variant; BA.2 Omicron variant; BA.4/BA.5 Omicron variant) (coated at 2 μg/mL) binding to soluble recombinant Human ACE2 (ECD) protein, mouse IgG Fc tag (active) (GTX135683-pro) (0.16-117.19 nM). Bound protein was detected by Goat Anti-Mouse IgG antibody (HRP) (GTX213111-01) (1:10000).
SARS-CoV-2 (COVID-19) Spike (ECD) Protein, Omicron / BA.1 variant, His tag
GTX136780-PRO
ApplicationsWestern Blot, ELISA, Other Application
Product group Proteins / Signaling Molecules
Overview
- SupplierGeneTex
- Product NameSARS-CoV-2 (COVID-19) Spike (ECD) Protein, Omicron / BA.1 variant, His tag
- Delivery Days Customer9
- Application Supplier NoteRecommended antibody pairs for sandwich ELISA: Capture : GTX635807, Detection : GTX635792-01.
- ApplicationsWestern Blot, ELISA, Other Application
- CertificationResearch Use Only
- ConjugateUnconjugated
- Storage Instruction-20°C,-80°C,2°C to 8°C
- UNSPSC41116100
- SpeciesVirus