
Indirect ELISA analysis was performed by coating the plate with recombinant SARS-CoV-2 (COVID-19) Spike (ECD) Protein, Omicron / BA.4 / BA.5 variant, His tag (GTX137113-pro) (28.78-0.45 nM). Coated protein was probed with the specified SARS-CoV-2 (COVID-19) Spike S1 antibodies (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) or goat anti-mouse IgG antibody (HRP) (GTX213111-01) (1:10000) were used to detect the bound primary antibodies.
SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1004]
GTX635793
ApplicationsImmunoFluorescence, ELISA, ImmunoCytoChemistry, Neutralisation/Blocking
Product group Antibodies
ReactivityVirus
Overview
- SupplierGeneTex
- Product NameSARS-CoV-2 (COVID-19) Spike RBD antibody [HL1004]
- Delivery Days Customer9
- Application Supplier NoteICC/IF: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, ELISA, ImmunoCytoChemistry, Neutralisation/Blocking
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHL1004
- Concentration1 mg/ml
- ConjugateUnconjugated
- HostRabbit
- IsotypeIgG
- ReactivityVirus
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161

![Indirect ELISA analysis was performed by coating the plate with recombinant SARS-CoV-2 spike (ECD) trimer, omicron BA.2.75 variant, His tag (GTX137533-pro) (28.62-0.45 nM). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1004] (the specified SARS-CoV-2 (COVID-19) Spike antibodies) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody. Indirect ELISA analysis was performed by coating the plate with recombinant SARS-CoV-2 spike (ECD) trimer, omicron BA.2.75 variant, His tag (GTX137533-pro) (28.62-0.45 nM). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1004] (the specified SARS-CoV-2 (COVID-19) Spike antibodies) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX635793/GTX635793_44305_20220923_ELISA_Indirect_Multiple_22092622_117.webp)


![Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike (ECD) Protein, Omicron / BF.7 variant, His tag (GTX137880-pro) using antibodies as below. Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) (5 μg/mL) Detection: HRP-conjugated SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1004] (GTX635793) (1 μg/mL) Please notice that GTX635793 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635807. Please contact us for custom HRP-conjugated antibody. Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike (ECD) Protein, Omicron / BF.7 variant, His tag (GTX137880-pro) using antibodies as below. Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) (5 μg/mL) Detection: HRP-conjugated SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1004] (GTX635793) (1 μg/mL) Please notice that GTX635793 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635807. Please contact us for custom HRP-conjugated antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX635793/GTX635793_44251_20220113_ELISA_PAIR_23011618_142.webp)
![Sandwich ELISA detection of recombinant full-length SARS-CoV-2 spike (trimer) protein using antibodies as below. Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1004] (GTX635793) (5 μg/mL) Detection: HRP-conjugated SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) (1 μg/mL) Please notice that GTX635807 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635793. Please contact us for custom HRP-conjugated antibody. Sandwich ELISA detection of recombinant full-length SARS-CoV-2 spike (trimer) protein using antibodies as below. Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1004] (GTX635793) (5 μg/mL) Detection: HRP-conjugated SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) (1 μg/mL) Please notice that GTX635807 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635793. Please contact us for custom HRP-conjugated antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX635793/GTX635793_44963_20230303_ELISA_Sandwich_23030717_639.webp)
![Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) (GTX01546-pro) (50 ng). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1004] (GTX635793) (1000-1.19*10 -4 μM). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody. EC50 : 0.2 μM Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) (GTX01546-pro) (50 ng). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1004] (GTX635793) (1000-1.19*10 -4 μM). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody. EC50 : 0.2 μM](https://www.genetex.com/upload/website/prouct_img/normal/GTX635793/GTX635793_44130_20210312_ELISA_Indirect_EC50_w_23061202_498.webp)
![Sandwich ELISA detection of recombinant Spike S1 proteins derived from different strains of SARS-CoV-2 virus (Wild type or B1.617.2 delta variant) using antibodies as below.
Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) (5 μg/mL)
Detection: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1004] (HRP) (GTX635793-01) (1 μg/mL). Sandwich ELISA detection of recombinant Spike S1 proteins derived from different strains of SARS-CoV-2 virus (Wild type or B1.617.2 delta variant) using antibodies as below.
Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) (5 μg/mL)
Detection: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1004] (HRP) (GTX635793-01) (1 μg/mL).](https://www.genetex.com/upload/website/prouct_img/normal/GTX635793/GTX635793_44251_20210728_ELISA_PAIR_Multiple_w_23061202_830.webp)
![Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) Spike RBD (L452R, E484Q Mutant) protein, His tag (active) (Indian variant) (GTX136299-pro) and SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) (Wild type) (GTX136090-pro) (4000-62.5 ng/mL). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1004] (GTX635793) (1 μg/mL). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody. Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) Spike RBD (L452R, E484Q Mutant) protein, His tag (active) (Indian variant) (GTX136299-pro) and SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) (Wild type) (GTX136090-pro) (4000-62.5 ng/mL). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1004] (GTX635793) (1 μg/mL). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX635793/GTX635793_44305_20210624_ELISA_Indirect_w_23061202_413.webp)