Functional ELISA analysis of immobilized recombinant SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) (GTX01546-pro) (coated at 2 microg/mL) binding to soluble recombinant Human ACE2 (ECD) protein , mouse IgG Fc tag (GTX135683-pro) (4.5-10000 ng/mL). Bound protein was detected by ACE2 antibody [N1N2], N-term (GTX101395) (0.5 microg/mL) and Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) detected bound primary antibody.
![Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) (GTX01546-pro) and SARS-CoV-2 (COVID-19) Spike RBD (K417N, E484K, N501Y Mutant) protein, His tag (active) (GTX136022-pro) using SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) as capture antibody at concentration of 5 microg/mL and HRP-conjugated SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1004] (GTX635793) as detection antibody at concentration of 1 microg/mL. Please notice that GTX635793 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635807. Please contact us for custom HRP-conjugated antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX01546-pro/GTX01546-pro_20210219_ELISA_PAIR_w_23053121_582.webp "Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) (GTX01546-pro) and SARS-CoV-2 (COVID-19) Spike RBD (K417N, E484K, N501Y Mutant) protein, His tag (active) (GTX136022-pro) using SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) as capture antibody at concentration of 5 microg/mL and HRP-conjugated SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1004] (GTX635793) as detection antibody at concentration of 1 microg/mL. Please notice that GTX635793 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635807. Please contact us for custom HRP-conjugated antibody.")
![Inhibition analysis of immobilized recombinant SARS-CoV-2 (COVID-19) Spike RBD (N501Y Mutant) protein, His tag (active) (GTX136014-pro), SARS-CoV-2 (COVID-19) Spike RBD (K417N, E484K, N501Y Mutant) protein, His tag (active) (GTX136022-pro), and SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) (GTX01546-pro) (coated at 2 microg/mL) binding to soluble recombinant Human ACE2 (ECD) protein , mouse IgG Fc tag (GTX135683-pro) (1000 ng/mL). ACE2 binding was inhibited by increasing concentrations of SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] (GTX635792) (14-10000 ng/mL). Bound ACE2 protein was detected by Goat Anti-Mouse IgG antibody (HRP) (GTX213111-01) (1:10000).](https://www.genetex.com/upload/website/prouct_img/normal/GTX01546-pro/GTX01546-pro_822100687_20210226_Neutralizing_Inhibition_w_23053121_754.webp "Inhibition analysis of immobilized recombinant SARS-CoV-2 (COVID-19) Spike RBD (N501Y Mutant) protein, His tag (active) (GTX136014-pro), SARS-CoV-2 (COVID-19) Spike RBD (K417N, E484K, N501Y Mutant) protein, His tag (active) (GTX136022-pro), and SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) (GTX01546-pro) (coated at 2 microg/mL) binding to soluble recombinant Human ACE2 (ECD) protein , mouse IgG Fc tag (GTX135683-pro) (1000 ng/mL). ACE2 binding was inhibited by increasing concentrations of SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] (GTX635792) (14-10000 ng/mL). Bound ACE2 protein was detected by Goat Anti-Mouse IgG antibody (HRP) (GTX213111-01) (1:10000).")
![Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike RBD (N501Y Mutant) protein, His tag (active) (GTX136014-pro), SARS-CoV-2 (COVID-19) Spike RBD (K417N, E484K, N501Y Mutant) protein, His tag (active) (GTX136022-pro), and SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) (GTX01546-pro) using SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) as capture antibody at concentration of 5 microg/mL and SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] (HRP) (GTX635792-01) as detection antibody at concentration of 1 microg/mL.](https://www.genetex.com/upload/website/prouct_img/normal/GTX01546-pro/GTX01546-pro_822100687_20210305_ELISA_PAIR_2_w_23053121_642.webp "Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike RBD (N501Y Mutant) protein, His tag (active) (GTX136014-pro), SARS-CoV-2 (COVID-19) Spike RBD (K417N, E484K, N501Y Mutant) protein, His tag (active) (GTX136022-pro), and SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) (GTX01546-pro) using SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) as capture antibody at concentration of 5 microg/mL and SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] (HRP) (GTX635792-01) as detection antibody at concentration of 1 microg/mL.")
![Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike RBD (N501Y Mutant) protein, His tag (active) (GTX136014-pro), SARS-CoV-2 (COVID-19) Spike RBD (K417N, E484K, N501Y Mutant) protein, His tag (active) (GTX136022-pro), and SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) (GTX01546-pro) using SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) as capture antibody at concentration of 5 microg/mL and SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1004] (HRP) (GTX635793-01) as detection antibody at concentration of 1 microg/mL.](https://www.genetex.com/upload/website/prouct_img/normal/GTX01546-pro/GTX01546-pro_822100687_20210305_ELISA_PAIR_w_23053121_362.webp "Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike RBD (N501Y Mutant) protein, His tag (active) (GTX136014-pro), SARS-CoV-2 (COVID-19) Spike RBD (K417N, E484K, N501Y Mutant) protein, His tag (active) (GTX136022-pro), and SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) (GTX01546-pro) using SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) as capture antibody at concentration of 5 microg/mL and SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1004] (HRP) (GTX635793-01) as detection antibody at concentration of 1 microg/mL.")
![Inhibition analysis of immobilized recombinant SARS-CoV-2 (COVID-19) Spike RBD (N501Y Mutant) protein, His tag (active) (GTX136014-pro), SARS-CoV-2 (COVID-19) Spike RBD (K417N, E484K, N501Y Mutant) protein, His tag (active) (GTX136022-pro), and SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) (GTX01546-pro) (coated at 2 microg/mL) binding to soluble recombinant Human ACE2 (ECD) protein , mouse IgG Fc tag (GTX135683-pro) (1000 ng/mL). ACE2 binding was inhibited by increasing concentrations of SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1004] (GTX635793) (14-10000 ng/mL). Bound ACE2 protein was detected by Goat Anti-Mouse IgG antibody (HRP) (13111-01) (1:10000).](https://www.genetex.com/upload/website/prouct_img/normal/GTX01546-pro/GTX01546-pro_822100687_20210305_Neutralizing_Inhibition_w_23053121_630.webp "Inhibition analysis of immobilized recombinant SARS-CoV-2 (COVID-19) Spike RBD (N501Y Mutant) protein, His tag (active) (GTX136014-pro), SARS-CoV-2 (COVID-19) Spike RBD (K417N, E484K, N501Y Mutant) protein, His tag (active) (GTX136022-pro), and SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) (GTX01546-pro) (coated at 2 microg/mL) binding to soluble recombinant Human ACE2 (ECD) protein , mouse IgG Fc tag (GTX135683-pro) (1000 ng/mL). ACE2 binding was inhibited by increasing concentrations of SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1004] (GTX635793) (14-10000 ng/mL). Bound ACE2 protein was detected by Goat Anti-Mouse IgG antibody (HRP) (13111-01) (1:10000).")
![Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) (GTX01546-pro) (50 ng). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1004] (GTX635793) (1000-1.19*10-4 microM). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody. EC50 : 0.2 microM](https://www.genetex.com/upload/website/prouct_img/normal/GTX01546-pro/GTX01546-pro_822100687_20210312_ELISA_Indirect_EC50_2_w_23053121_799.webp "Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) (GTX01546-pro) (50 ng). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1004] (GTX635793) (1000-1.19*10-4 microM). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody. EC50 : 0.2 microM")
![Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) (GTX01546-pro) (50 ng). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) (1000-1.19*10-4 microM). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody. EC50 : 0.24 microM](https://www.genetex.com/upload/website/prouct_img/normal/GTX01546-pro/GTX01546-pro_822100687_20210312_ELISA_Indirect_EC50_3_w_23053121_769.webp "Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) (GTX01546-pro) (50 ng). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) (1000-1.19*10-4 microM). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody. EC50 : 0.24 microM")
![Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) (GTX01546-pro) (50 ng). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] (GTX635792) (160-1.91*10-5 microM). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody. EC50 : 0.08 microM](https://www.genetex.com/upload/website/prouct_img/normal/GTX01546-pro/GTX01546-pro_822100687_20210312_ELISA_Indirect_EC50_w_23053121_576.webp "Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) (GTX01546-pro) (50 ng). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] (GTX635792) (160-1.91*10-5 microM). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody. EC50 : 0.08 microM")
Functional ELISA analysis of immobilized recombinant SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) (GTX01546-pro) (coated at 2 microg/mL) binding to soluble recombinant Human ACE2 (ECD) protein , mouse IgG Fc tag (GTX135683-pro) (4.5-10000 ng/mL). Bound protein was detected by ACE2 antibody [N1N2], N-term (GTX101395) (0.5 microg/mL) and Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) detected bound primary antibody.
SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active)
GTX01546-PRO
ApplicationsFunctional Assay, ELISA
Product group Proteins / Signaling Molecules
Overview
- SupplierGeneTex
- Product NameSARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active)
- Delivery Days Customer9
- Application Supplier NoteActivity was measured by its binding ability in a functional ELISA. Immobilized Recombinant SARS-CoV-2 (COVID-19) Spike RBD protein at 2 microg/mL (100 microL/well) can bind Recombinant Human ACE2 with a linear range of 8-80 ng/mL.
- ApplicationsFunctional Assay, ELISA
- CertificationResearch Use Only
- ConjugateUnconjugated
- Storage Instruction-20°C
- UNSPSC12352202
References
- Liu TW, Hsu SJ, Hsieh YSY, et al. Polymethoxyflavone from Citrus depressa as an inhibitor against various variants of SARS-CoV-2 spike protein. J Ethnopharmacol. 2024,320:117412. doi: 10.1016/j.jep.2023.117412Read this paper
- Ganneru B, Jogdand H, Daram VK, et al. Th1 skewed immune response of whole virion inactivated SARS CoV 2 vaccine and its safety evaluation. iScience. 2021,24(4):102298. doi: 10.1016/j.isci.2021.102298Read this paper