![Sandwich ELISA detection of recombinant Spike RBD Protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; BA.1 Omicron variant; BA.2 Omicron variant; BA.4/ BA.5 Omicron variant) using antibodies as below. Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) (5 μg/mL) Detection: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1004] (HRP) (GTX635793-01) (1 μg/mL) Sandwich ELISA detection of recombinant Spike RBD Protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; BA.1 Omicron variant; BA.2 Omicron variant; BA.4/ BA.5 Omicron variant) using antibodies as below. Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) (5 μg/mL) Detection: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1004] (HRP) (GTX635793-01) (1 μg/mL)](https://www.genetex.com/upload/website/prouct_img/normal/GTX136090-pro/GTX136090-pro_P-44505_20220715_ELISA_PAIR_Multiple_22071823_896.webp)
Sandwich ELISA detection of recombinant Spike RBD Protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; BA.1 Omicron variant; BA.2 Omicron variant; BA.4/ BA.5 Omicron variant) using antibodies as below. Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) (5 μg/mL) Detection: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1004] (HRP) (GTX635793-01) (1 μg/mL)
SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active)
GTX136090-PRO
ApplicationsWestern Blot, ELISA, Other Application
Product group Proteins / Signaling Molecules
Overview
- SupplierGeneTex
- Product NameSARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active)
- Delivery Days Customer9
- Application Supplier NoteRecommended antibody pairs for sandwich ELISA: Capture: GTX635807, Detection: GTX635791-01.
- ApplicationsWestern Blot, ELISA, Other Application
- CertificationResearch Use Only
- Concentration0.7 mg/ml
- ConjugateUnconjugated
- Storage Instruction-20°C,-80°C
- UNSPSC41116100
- SpeciesVirus
![Sandwich ELISA detection of recombinant Spike RBD Protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; BA.2.12.1 Omicron variant) using antibodies as below. Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) (5 μg/mL) Detection: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] (HRP) (GTX635792-01) (1 μg/mL) Sandwich ELISA detection of recombinant Spike RBD Protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; BA.2.12.1 Omicron variant) using antibodies as below. Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) (5 μg/mL) Detection: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] (HRP) (GTX635792-01) (1 μg/mL)](https://www.genetex.com/upload/website/prouct_img/normal/GTX136090-pro/GTX136090-pro_P-44505_20220729_ELISA_PAIR_Multiple_22080119_887.webp)
![Sandwich ELISA detection of recombinant Spike RBD Protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; BA.2.75 Omicron variant) using antibodies as below. Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) (5 μg/mL) Detection: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] (HRP) (GTX635792-01) (1 μg/mL) Sandwich ELISA detection of recombinant Spike RBD Protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; BA.2.75 Omicron variant) using antibodies as below. Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) (5 μg/mL) Detection: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] (HRP) (GTX635792-01) (1 μg/mL)](https://www.genetex.com/upload/website/prouct_img/normal/GTX136090-pro/GTX136090-pro_P-44505_20220908_ELISA_PAIR_Multiple_22091323_911.webp)

![Inhibition analysis of immobilized recombinant RBD proteins derived from different strains of SARS-CoV-2 virus (Wild type or B1.617.2 delta variant) (coated at 2 μg/mL) binding to soluble recombinant Human ACE2 (ECD) protein, mouse IgG Fc tag (active) (GTX135683-pro) (1000 ng/mL) ACE2 binding was inhibited by increasing concentrations of SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1002] (GTX635791) (0.09-66.67 nM). Bound ACE2 was detected by Goat Anti-Mouse IgG antibody (HRP) (GTX213111-01) (1:10000). Inhibition analysis of immobilized recombinant RBD proteins derived from different strains of SARS-CoV-2 virus (Wild type or B1.617.2 delta variant) (coated at 2 μg/mL) binding to soluble recombinant Human ACE2 (ECD) protein, mouse IgG Fc tag (active) (GTX135683-pro) (1000 ng/mL) ACE2 binding was inhibited by increasing concentrations of SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1002] (GTX635791) (0.09-66.67 nM). Bound ACE2 was detected by Goat Anti-Mouse IgG antibody (HRP) (GTX213111-01) (1:10000).](https://www.genetex.com/upload/website/prouct_img/normal/GTX136090-pro/GTX136090-pro_44281_20210728_Neutralizing_Inhibition_Multiple_w_23060620_621.webp)

![Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) Spike RBD (L452R, E484Q Mutant) protein, His tag (active) (Indian variant) (GTX136299-pro) and SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) (Wild type) (GTX136090-pro) (4000-62.5 ng/mL). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1002] (GTX635791) (1 μg/mL). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody. Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) Spike RBD (L452R, E484Q Mutant) protein, His tag (active) (Indian variant) (GTX136299-pro) and SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) (Wild type) (GTX136090-pro) (4000-62.5 ng/mL). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1002] (GTX635791) (1 μg/mL). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX136090-pro/GTX136090-pro_44281_20210624_ELISA_Indirect_w_23060620_912.webp)
![Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike RBD (L452R, E484Q Mutant) protein, His tag (active) (Indian variant) (GTX136299-pro) and SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) (Wild type) (GTX136090-pro) using SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) as capture antibody at concentration of 5 μg/mL and HRP-conjugated SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1002] (GTX635791) as detection antibody at concentration of 1 μg/mL. Please notice that GTX635791 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635807. Please contact us for custom HRP-conjugated antibody. Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike RBD (L452R, E484Q Mutant) protein, His tag (active) (Indian variant) (GTX136299-pro) and SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) (Wild type) (GTX136090-pro) using SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) as capture antibody at concentration of 5 μg/mL and HRP-conjugated SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1002] (GTX635791) as detection antibody at concentration of 1 μg/mL. Please notice that GTX635791 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635807. Please contact us for custom HRP-conjugated antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX136090-pro/GTX136090-pro_44281_20210624_ELISA_PAIR_w_23060620_368.webp)

![Inhibition analysis of immobilized recombinant SARS-CoV-2 (COVID-19) Spike RBD Protein, AY.1 / Delta plus variant, His tag (delta plus variant) (GTX136333-pro) and SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) (wild type) (GTX136090-pro) (coated at 2 μg/mL) binding to soluble recombinant Human ACE2 (ECD) protein, mouse IgG Fc tag (active) (GTX135683-pro) (1000 ng/mL) ACE2 binding was inhibited by increasing concentrations of SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1002] (GTX635791) (0.09-66.67 nM). Bound ACE2 was detected by Goat Anti-Mouse IgG antibody (HRP) (GTX213111-01) (1:10000). Inhibition analysis of immobilized recombinant SARS-CoV-2 (COVID-19) Spike RBD Protein, AY.1 / Delta plus variant, His tag (delta plus variant) (GTX136333-pro) and SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) (wild type) (GTX136090-pro) (coated at 2 μg/mL) binding to soluble recombinant Human ACE2 (ECD) protein, mouse IgG Fc tag (active) (GTX135683-pro) (1000 ng/mL) ACE2 binding was inhibited by increasing concentrations of SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1002] (GTX635791) (0.09-66.67 nM). Bound ACE2 was detected by Goat Anti-Mouse IgG antibody (HRP) (GTX213111-01) (1:10000).](https://www.genetex.com/upload/website/prouct_img/normal/GTX136090-pro/GTX136090-pro_44281_20210813_Neutralizing_Inhibition_Multiple_w_23060620_634.webp)