Indirect ELISA analysis was performed by coating the plate with recombinant SARS-CoV-2 (COVID-19) Spike (ECD) Protein, Omicron / BA.4 / BA.5 variant, His tag (GTX137113-pro) (28.78-0.45 nM). Coated protein was probed with the specified SARS-CoV-2 (COVID-19) Spike S2 antibodies (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) or goat anti-mouse IgG antibody (HRP) (GTX213111-01) (1:10000) were used to detect the bound primary antibodies.
![Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) Spike S2 antibody [GT8110] (GTX636038) diluted at 1:1000000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX636038/GTX636038_44258_20210426_WB_SARScoronavirus2_B_w_23061202_462.webp "Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) Spike S2 antibody [GT8110] (GTX636038) diluted at 1:1000000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.")
![SARS-CoV-2 (COVID-19) Spike S2 antibody [GT8110] detects SARS-CoV-2 (COVID-19) Spike S2 protein at cytoplasm by immunohistochemical analysis. Sample: Mock (GTX435670) and SARS-CoV-2 (COVID-19) Spike transfected 293T cell FFPE Cell Pellet Block (GTX435640). Green: SARS-CoV-2 (COVID-19) Spike S2 stained by SARS-CoV-2 (COVID-19) Spike S2 antibody [GT8110] (GTX636038) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920). Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX636038/GTX636038_44258_20210625_IHC-P_B_w_23061202_661.webp "SARS-CoV-2 (COVID-19) Spike S2 antibody [GT8110] detects SARS-CoV-2 (COVID-19) Spike S2 protein at cytoplasm by immunohistochemical analysis. Sample: Mock (GTX435670) and SARS-CoV-2 (COVID-19) Spike transfected 293T cell FFPE Cell Pellet Block (GTX435640). Green: SARS-CoV-2 (COVID-19) Spike S2 stained by SARS-CoV-2 (COVID-19) Spike S2 antibody [GT8110] (GTX636038) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920). Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Sandwich ELISA detection of recombinant SARS-CoV-2 spike (trimer) protein using SARS-CoV-2 (COVID-19) Spike S2 antibody [GT8110] (GTX636038) as capture antibody at concentration of 5 μg/mL and SARS-CoV-2 (COVID-19) Spike S2 antibody [HL1038] (GTX635910) as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX636038/GTX636038_44258_20210423_ELISA_PAIR_2_w_23061202_499.webp "Sandwich ELISA detection of recombinant SARS-CoV-2 spike (trimer) protein using SARS-CoV-2 (COVID-19) Spike S2 antibody [GT8110] (GTX636038) as capture antibody at concentration of 5 μg/mL and SARS-CoV-2 (COVID-19) Spike S2 antibody [HL1038] (GTX635910) as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.")
![Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike S2 (ECD) protein, human IgG Fc tag (GTX01559-pro) using SARS-CoV-2 (COVID-19) Spike S2 antibody [GT8110] (GTX636038) as capture antibody at concentration of 5 μg/mL and SARS-CoV-2 (COVID-19) Spike S2 antibody [HL1038] (GTX635910) as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX636038/GTX636038_44258_20210423_ELISA_PAIR_w_23061202_625.webp "Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike S2 (ECD) protein, human IgG Fc tag (GTX01559-pro) using SARS-CoV-2 (COVID-19) Spike S2 antibody [GT8110] (GTX636038) as capture antibody at concentration of 5 μg/mL and SARS-CoV-2 (COVID-19) Spike S2 antibody [HL1038] (GTX635910) as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.")
Indirect ELISA analysis was performed by coating the plate with recombinant SARS-CoV-2 (COVID-19) Spike (ECD) Protein, Omicron / BA.4 / BA.5 variant, His tag (GTX137113-pro) (28.78-0.45 nM). Coated protein was probed with the specified SARS-CoV-2 (COVID-19) Spike S2 antibodies (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) or goat anti-mouse IgG antibody (HRP) (GTX213111-01) (1:10000) were used to detect the bound primary antibodies.
SARS-CoV-2 (COVID-19) Spike S2 antibody [GT8110]
GTX636038
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityVirus
Overview
- SupplierGeneTex
- Product NameSARS-CoV-2 (COVID-19) Spike S2 antibody [GT8110]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:5000-1:1000000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDGT8110
- Concentration1 mg/ml
- ConjugateUnconjugated
- HostMouse
- IsotypeIgG2a
- ReactivityVirus
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161