Indirect ELISA analysis was performed by coating the plate with recombinant SARS-CoV-2 (COVID-19) Spike (ECD) Protein, Omicron / BA.4 / BA.5 variant, His tag (GTX137113-pro) (28.78-0.45 nM). Coated protein was probed with the specified SARS-CoV-2 (COVID-19) Spike S2 antibodies (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) or goat anti-mouse IgG antibody (HRP) (GTX213111-01) (1:10000) were used to detect the bound primary antibodies.
![Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike (ECD) protein, His tag (active) (GTX135972-pro) using antibodies as below. Capture: SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) (5 μg/mL) Detection: SARS-CoV-2 (COVID-19) Spike S2 antibody [HL1038] (GTX635910) (1 μg/mL)](https://www.genetex.com/upload/website/prouct_img/normal/GTX635910/GTX635910_44858_20221111_ELISA_PAIR_22112219_226.webp "Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike (ECD) protein, His tag (active) (GTX135972-pro) using antibodies as below. Capture: SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) (5 μg/mL) Detection: SARS-CoV-2 (COVID-19) Spike S2 antibody [HL1038] (GTX635910) (1 μg/mL)")
![Sandwich ELISA detection of recombinant SARS-CoV-2 spike (trimer) protein using SARS-CoV-2 (COVID-19) Spike S2 antibody [GT745] (GTX636037) as capture antibody at concentration of 5 μg/mL and SARS-CoV-2 (COVID-19) Spike S2 antibody [HL1038] (GTX635910) as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX635910/GTX635910_44235_20210423_ELISA_PAIR_3_w_23061202_121.webp "Sandwich ELISA detection of recombinant SARS-CoV-2 spike (trimer) protein using SARS-CoV-2 (COVID-19) Spike S2 antibody [GT745] (GTX636037) as capture antibody at concentration of 5 μg/mL and SARS-CoV-2 (COVID-19) Spike S2 antibody [HL1038] (GTX635910) as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.")
![SARS-CoV-2 (COVID-19) Spike ( L18F,..., K417N, E484K, N501Y,...)(ECD) Protein, His tag (active) (GTX136061-pro, 0.2 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) Spike S2 antibody [HL1038] (GTX635910) diluted at 1:150000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX635910/GTX635910_44048_20210319_WB_SARScoronavirus2_Ag_w_23061202_480.webp "SARS-CoV-2 (COVID-19) Spike ( L18F,..., K417N, E484K, N501Y,...)(ECD) Protein, His tag (active) (GTX136061-pro, 0.2 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) Spike S2 antibody [HL1038] (GTX635910) diluted at 1:150000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike S2 (T716I, S982A, D1118H Mutant) (ECD) protein, His tag (GTX136023-pro), SARS-CoV-2 (COVID-19) Spike S2 (ECD) protein, mouse IgG Fc tag (GTX135684-pro), and SARS-CoV-2 (COVID-19) Spike S2 (ECD) protein, human IgG Fc tag (GTX01559-pro) using SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) as capture antibody at concentration of 5 μg/mL and SARS-CoV-2 (COVID-19) Spike S2 antibody [HL1038] (GTX635910) as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX635910/GTX635910_T-44179_20210312_ELISA_PAIR_w_23061202_766.webp "Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike S2 (T716I, S982A, D1118H Mutant) (ECD) protein, His tag (GTX136023-pro), SARS-CoV-2 (COVID-19) Spike S2 (ECD) protein, mouse IgG Fc tag (GTX135684-pro), and SARS-CoV-2 (COVID-19) Spike S2 (ECD) protein, human IgG Fc tag (GTX01559-pro) using SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) as capture antibody at concentration of 5 μg/mL and SARS-CoV-2 (COVID-19) Spike S2 antibody [HL1038] (GTX635910) as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.")
![SARS-CoV-2 (COVID-19) Spike S2 antibody [HL1038] detects SARS-CoV-2 (COVID-19) Spike S2 protein at cytoplasm by immunofluorescent analysis. Sample: Mock and transfected SARS-CoV-2 (COVID-19) Spike transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SARS-CoV-2 (COVID-19) Spike S2 stained by SARS-CoV-2 (COVID-19) Spike S2 antibody [HL1038] (GTX635910) diluted at 1:1000.](https://www.genetex.com/upload/website/prouct_img/normal/GTX635910/GTX635910_T-44179_20210122_ICC_IF_w_23061202_164.webp "SARS-CoV-2 (COVID-19) Spike S2 antibody [HL1038] detects SARS-CoV-2 (COVID-19) Spike S2 protein at cytoplasm by immunofluorescent analysis. Sample: Mock and transfected SARS-CoV-2 (COVID-19) Spike transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SARS-CoV-2 (COVID-19) Spike S2 stained by SARS-CoV-2 (COVID-19) Spike S2 antibody [HL1038] (GTX635910) diluted at 1:1000.")
![SARS-CoV-2 (COVID-19) Spike S2 antibody [HL1038] detects SARS-CoV-2 (COVID-19) Spike S2 protein at cytoplasm by immunohistochemical analysis. Sample: Mock (GTX435670) and SARS-CoV-2 (COVID-19) Spike transfected 293T cell FFPE Cell Pellet Block (GTX435640). Green: SARS-CoV-2 (COVID-19) Spike S2 stained by SARS-CoV-2 (COVID-19) Spike S2 antibody [HL1038] (GTX635910) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920). Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX635910/GTX635910_44235_20210625_IHC-P_B_w_23061202_554.webp "SARS-CoV-2 (COVID-19) Spike S2 antibody [HL1038] detects SARS-CoV-2 (COVID-19) Spike S2 protein at cytoplasm by immunohistochemical analysis. Sample: Mock (GTX435670) and SARS-CoV-2 (COVID-19) Spike transfected 293T cell FFPE Cell Pellet Block (GTX435640). Green: SARS-CoV-2 (COVID-19) Spike S2 stained by SARS-CoV-2 (COVID-19) Spike S2 antibody [HL1038] (GTX635910) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920). Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Sandwich ELISA detection of recombinant SARS-CoV-2 spike (trimer) protein using SARS-CoV-2 (COVID-19) Spike S2 antibody [GT8110] (GTX636038) as capture antibody at concentration of 5 μg/mL and SARS-CoV-2 (COVID-19) Spike S2 antibody [HL1038] (GTX635910) as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX635910/GTX635910_44235_20210423_ELISA_PAIR_4_w_23061202_325.webp "Sandwich ELISA detection of recombinant SARS-CoV-2 spike (trimer) protein using SARS-CoV-2 (COVID-19) Spike S2 antibody [GT8110] (GTX636038) as capture antibody at concentration of 5 μg/mL and SARS-CoV-2 (COVID-19) Spike S2 antibody [HL1038] (GTX635910) as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.")
![Sandwich ELISA detection of recombinant SARS-CoV-2 spike (trimer) protein using antibodies as below.
Capture: SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) (5 μg/mL)
Detection: SARS-CoV-2 (COVID-19) Spike S2 antibody [HL1038] (GTX635910) (1 μg/mL).](https://www.genetex.com/upload/website/prouct_img/normal/GTX635910/GTX635910_44235_20210726_ELISA_PAIR_w_23061202_457.webp "Sandwich ELISA detection of recombinant SARS-CoV-2 spike (trimer) protein using antibodies as below.
Capture: SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) (5 μg/mL)
Detection: SARS-CoV-2 (COVID-19) Spike S2 antibody [HL1038] (GTX635910) (1 μg/mL).")
![SARS-CoV-2 (COVID-19) Spike S2 antibody [HL1038] detects SARS-CoV-2 (COVID-19) Spike S2 protein by immunofluorescent analysis. Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SARS-CoV-2 (COVID-19) Spike S2 stained by SARS-CoV-2 (COVID-19) Spike S2 antibody [HL1038] (GTX635910) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).](https://www.genetex.com/upload/website/prouct_img/normal/GTX635910/GTX635910_44606_20220429_ICC_IF_ICON02175_w_23061202_649.webp "SARS-CoV-2 (COVID-19) Spike S2 antibody [HL1038] detects SARS-CoV-2 (COVID-19) Spike S2 protein by immunofluorescent analysis. Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SARS-CoV-2 (COVID-19) Spike S2 stained by SARS-CoV-2 (COVID-19) Spike S2 antibody [HL1038] (GTX635910) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).")
Indirect ELISA analysis was performed by coating the plate with recombinant SARS-CoV-2 (COVID-19) Spike (ECD) Protein, Omicron / BA.4 / BA.5 variant, His tag (GTX137113-pro) (28.78-0.45 nM). Coated protein was probed with the specified SARS-CoV-2 (COVID-19) Spike S2 antibodies (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) or goat anti-mouse IgG antibody (HRP) (GTX213111-01) (1:10000) were used to detect the bound primary antibodies.
SARS-CoV-2 (COVID-19) Spike S2 antibody [HL1038]
GTX635910
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityVirus
Overview
- SupplierGeneTex
- Product NameSARS-CoV-2 (COVID-19) Spike S2 antibody [HL1038]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:5000-1:100000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHL1038
- Concentration1 mg/ml
- ConjugateUnconjugated
- HostRabbit
- IsotypeIgG
- ReactivityVirus
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161