SARS-CoV NSP13 Antibody (aa584-601)
LS-C59285
ApplicationsImmunoFluorescence
Product group Antibodies
ReactivityVirus
Overview
- SupplierLifeSpan BioSciences
- Product NameSARS-CoV NSP13 Antibody (aa584-601)
- Delivery Days Customer23
- Antibody SpecificityThis antibody is directed against SARS-Coronavirus nsp13 protein. The product is neat antiserum. Cross reactivity with homologues from other sources has not been determined.
- Application Supplier NoteFor immunofluorescence microscopy, Vero-E6 cells, grown on glass slides, were infected with SARS-CoV-Fr1 strain for 1 h at 37?C. Infection occurred in PBS/DEAE/2%FCS followed by exchange to EMEM/25mMHEPES/2%FCS. Cells were fixed with PBS/3%PFA. After washing fixed cells, antibody incubation was performed in PBS/5%FCS for 30 min.. IF (1:800) For immunofluorescence microscopy, Vero-E6 cells, grown on glass slides, were infected with SARS-CoV-Fr1 strain for 1 h at 37?C. Infection occurred in PBS/DEAE/2%FCS followed by exchange to EMEM/25mMHEPES/2%FCS. Cells were fixed with PBS/3%PFA. After washing fixed cells, antibody incubation was performed in PBS/5%FCS for 30 min.
- ApplicationsImmunoFluorescence
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration85 mg/ml
- ConjugateUnconjugated
- HostRabbit
- ReactivityVirus
- Storage Instruction-20°C
- UNSPSC12352203