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SARS-CoV NSP8 Antibody

Research Use Only
LS-C59284
LifeSpan BioSciences
ApplicationsElectron Microscopy, ImmunoFluorescence, ImmunoPrecipitation, Western Blot
Product group Antibodies
ReactivityVirus
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    SARS-CoV NSP8 Antibody
  • Delivery Days Customer
    23
  • Antibody Specificity
    This antibody is directed against SARS-Coronavirus nsp8 protein. The product is neat antiserum. Cross reactivity with homologues from other sources has not been determined.
  • Application Supplier Note
    This antibody has been tested for use in immunofluorescence microscopy, immunoelectron microscopy, immunoprecipitation and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 22 kD in size corresponding to SARS-CoV nsp8 by western blotting in the appropriate cell lysate or extract. For immunofluorescence microscopy, Vero-E6 cells, grown on glass slides, were infected with SARS-CoV-Fr1 strain for 1 h at 37?C. Infection occurred in PBS/DEAE/2%FCS followed by exchange to EMEM/25mMHEPES/2%FCS. Cells were fixed with PBS/3%PFA. After washing fixed cells, antibody incubation was performed in PBS/5%FCS for 30 min. IMMUNO PRECIPITATION 1:60; IMMUNO ELECTRON MICROSCOPY 1:100;. EM, IF (1:300), IP (1:60), WB (1:1000) This antibody has been tested for use in immunofluorescence microscopy, immunoelectron microscopy, immunoprecipitation and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 22 kD in size corresponding to SARS-CoV nsp8 by western blotting in the appropriate cell lysate or extract. For immunofluorescence microscopy, Vero-E6 cells, grown on glass slides, were infected with SARS-CoV-Fr1 strain for 1 h at 37?C. Infection occurred in PBS/DEAE/2%FCS followed by exchange to EMEM/25mMHEPES/2%FCS. Cells were fixed with PBS/3%PFA. After washing fixed cells, antibody incubation was performed in PBS/5%FCS for 30 min. IMMUNO PRECIPITATION 1:60; IMMUNO ELECTRON MICROSCOPY 1:100;
  • Applications
    Electron Microscopy, ImmunoFluorescence, ImmunoPrecipitation, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    85 mg/ml
  • Conjugate
    Unconjugated
  • Host
    Rabbit
  • Reactivity
    Virus
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203