![SARS-CoV / SARS-CoV-2 (COVID-19) NSP8 antibody [5A10] (GTX632696) detects SARS-CoV-2 (COVID-19) NSP8 by flow cytometry analysis. Sample: Vero E6 cells infected with SARS-CoV-2. Black: Uninfected Vero E6 cells was used as a control. Red: SARS-CoV / SARS-CoV-2 (COVID-19) NSP8 antibody [5A10] (GTX632696) dilution: 1:100. SARS-CoV / SARS-CoV-2 (COVID-19) NSP8 antibody [5A10] (GTX632696) detects SARS-CoV-2 (COVID-19) NSP8 by flow cytometry analysis. Sample: Vero E6 cells infected with SARS-CoV-2. Black: Uninfected Vero E6 cells was used as a control. Red: SARS-CoV / SARS-CoV-2 (COVID-19) NSP8 antibody [5A10] (GTX632696) dilution: 1:100.](https://www.genetex.com/upload/website/prouct_img/normal/GTX632696/GTX632696_43969_20200714_FACS_SARS-CoV-2_curstmerfeedback_w_23061202_870.webp)
SARS-CoV / SARS-CoV-2 (COVID-19) NSP8 antibody [5A10] (GTX632696) detects SARS-CoV-2 (COVID-19) NSP8 by flow cytometry analysis. Sample: Vero E6 cells infected with SARS-CoV-2. Black: Uninfected Vero E6 cells was used as a control. Red: SARS-CoV / SARS-CoV-2 (COVID-19) NSP8 antibody [5A10] (GTX632696) dilution: 1:100.
SARS-CoV / SARS-CoV-2 (COVID-19) nsp8 antibody [5A10]
GTX632696
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
Product group Antibodies
ReactivityVirus
Overview
- SupplierGeneTex
- Product NameSARS-CoV / SARS-CoV-2 (COVID-19) nsp8 antibody [5A10]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:1000-1:10000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID5A10
- Concentration0.67 mg/ml
- ConjugateUnconjugated
- HostMouse
- IsotypeIgG1
- ReactivityVirus
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Zhang X, Yang Z, Pan T, et al. SARS-CoV-2 Nsp8 suppresses MDA5 antiviral immune responses by impairing TRIM4-mediated K63-linked polyubiquitination. PLoS Pathog. 2023,19(11):e1011792. doi: 10.1371/journal.ppat.1011792Read this paper
- Guichard A, Lu S, Kanca O, et al. A comprehensive Drosophila resource to identify key functional interactions between SARS-CoV-2 factors and host proteins. Cell Rep. 2023,42(8):112842. doi: 10.1016/j.celrep.2023.112842Read this paper
- Hirano M, Ando R, Shimozono S, et al. A highly photostable and bright green fluorescent protein. Nat Biotechnol. 2022,40(7):1132-1142. doi: 10.1038/s41587-022-01278-2Read this paper
- Madden PJ, Thomas Y, Blair RV, et al. An immunoPET probe to SARS-CoV-2 reveals early infection of the male genital tract in rhesus macaques. Res Sq. 2022,:pii: rs.3.rs-1479315. doi: 10.21203/rs.3.rs-1479315/v1.Read this paper
- Madden PJ, Thomas Y, Blair RV, et al. An immunoPET probe to SARS-CoV-2 reveals early infection of the male genital tract in rhesus macaques. bioRxiv. 2022,:pii: 2022.02.25.481974. doi: 10.1101/2022.02.25.481974.Read this paper
- Ter Ellen BM, Dinesh Kumar N, Bouma EM, et al. Resveratrol and Pterostilbene Inhibit SARS-CoV-2 Replication in Air-Liquid Interface Cultured Human Primary Bronchial Epithelial Cells. Viruses. 2021,13(7). doi: 10.3390/v13071335Read this paper
- Ciccosanti F, Di Rienzo M, Romagnoli A, et al. Proteomic analysis identifies the RNA helicase DDX3X as a host target against SARS-CoV-2 infection. Antiviral Res. 2021,190:105064. doi: 10.1016/j.antiviral.2021.105064Read this paper
- Kotaki T, Xie X, Shi PY, et al. A PCR amplicon-based SARS-CoV-2 replicon for antiviral evaluation. Sci Rep. 2021,11(1):2229. doi: 10.1038/s41598-021-82055-0Read this paper
- Sauvat A, Ciccosanti F, Colavita F, et al. On-target versus off-target effects of drugs inhibiting the replication of SARS-CoV-2. Cell Death Dis. 2020,11(8):656. doi: 10.1038/s41419-020-02842-xRead this paper
![SARS-CoV / SARS-CoV-2 (COVID-19) NSP8 antibody [5A10] detects SARS-CoV / SARS-CoV-2 (COVID-19) NSP8 protein by immunofluorescent analysis. Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SARS-CoV / SARS-CoV-2 (COVID-19) NSP8 stained by SARS-CoV / SARS-CoV-2 (COVID-19) NSP8 antibody [5A10] (GTX632696) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920). SARS-CoV / SARS-CoV-2 (COVID-19) NSP8 antibody [5A10] detects SARS-CoV / SARS-CoV-2 (COVID-19) NSP8 protein by immunofluorescent analysis. Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SARS-CoV / SARS-CoV-2 (COVID-19) NSP8 stained by SARS-CoV / SARS-CoV-2 (COVID-19) NSP8 antibody [5A10] (GTX632696) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920).](https://www.genetex.com/upload/website/prouct_img/normal/GTX632696/GTX632696_43969_20200708_ICC_IF_SARScoronavirus_B_w_23061202_409.webp)
![Non-infected (–) and infected (+, 48h pl MOI 0.01) whole cell extracts were separated by SDS-PAGE, and the membrane was blotted with SARS-CoV / SARS-CoV-2 (COVID-19) NSP8 antibody [5A10] (GTX632696) diluted at 1:1000. Non-infected (–) and infected (+, 48h pl MOI 0.01) whole cell extracts were separated by SDS-PAGE, and the membrane was blotted with SARS-CoV / SARS-CoV-2 (COVID-19) NSP8 antibody [5A10] (GTX632696) diluted at 1:1000.](https://www.genetex.com/upload/website/prouct_img/normal/GTX632696/GTX632696_42345_20200313_WB_SARScoronavirus_w_23061202_232.webp)
![Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with SARS-CoV / SARS-CoV-2 (COVID-19) NSP8 antibody [5A10] (GTX632696) diluted at 1:5000. Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with SARS-CoV / SARS-CoV-2 (COVID-19) NSP8 antibody [5A10] (GTX632696) diluted at 1:5000.](https://www.genetex.com/upload/website/prouct_img/normal/GTX632696/GTX632696_42345_20151231_WB_B_w_23061202_561.webp)
