SARS-CoV-2 (COVID-19) nucleocapsid protein (GTX135592-pro, 0.5 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] (GTX632269) diluted at 1:5000. The HRP-conjugated anti-mouset IgG antibody (GTX213111-01) was used to detect the primary antibody.
![Sandwich ELISA detection of non-transfected and SARS-CoV-2 nucleocapsid (full length) transfected 293T whole cell extracts using SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] (GTX632269) as capture antibody at concentration of 5 弮g/mL and SARS-CoV-2 (COVID-19) nucleocapsid antibody (GTX135357) as detection antibody at concentration of 1 弮g/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX632269/GTX632269_43936_20200605_ELISA_PAIR_B_w_23061202_385.webp "Sandwich ELISA detection of non-transfected and SARS-CoV-2 nucleocapsid (full length) transfected 293T whole cell extracts using SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] (GTX632269) as capture antibody at concentration of 5 弮g/mL and SARS-CoV-2 (COVID-19) nucleocapsid antibody (GTX135357) as detection antibody at concentration of 1 弮g/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.")
![Immunoprecipitation of SARS-CoV-2 NP transfected 293T whole cell extracts using 2 μg of SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] (GTX632269). Western blot analysis was performed using SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] (GTX632269). EasyBlot HRP-conjugated anti mouse IgG antibody (GTX221667-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX632269/GTX632269_42079_20200417_IP_B_w_23061202_484.webp "Immunoprecipitation of SARS-CoV-2 NP transfected 293T whole cell extracts using 2 μg of SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] (GTX632269). Western blot analysis was performed using SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] (GTX632269). EasyBlot HRP-conjugated anti mouse IgG antibody (GTX221667-01) was used to detect the primary antibody.")
![SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] detects SARS-CoV-2 (COVID-19) nucleocapsid protein by immunohistochemical analysis. Sample: Mock (GTX435670) and SARS-CoV-2 (COVID-19) Nucleocapsid transfected 293T cell FFPE Cell Pellet Block (GTX435641). Red: SARS-CoV-2 (COVID-19) nucleocapsid stained by SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] (GTX632269) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920). Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX632269/GTX632269_43936_20200515_IHC-P-FL_B_w_23061202_426.webp "SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] detects SARS-CoV-2 (COVID-19) nucleocapsid protein by immunohistochemical analysis. Sample: Mock (GTX435670) and SARS-CoV-2 (COVID-19) Nucleocapsid transfected 293T cell FFPE Cell Pellet Block (GTX435641). Red: SARS-CoV-2 (COVID-19) nucleocapsid stained by SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] (GTX632269) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920). Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] (GTX632269) diluted at 1:5000. The HRP-conjugated anti-mouset IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](https://www.genetex.com/upload/website/prouct_img/normal/GTX632269/GTX632269_43936_20200526_WB_B_multiplevirus_w_23061202_516.webp "Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] (GTX632269) diluted at 1:5000. The HRP-conjugated anti-mouset IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] detects SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid protein by immunohistochemical analysis. Sample: Paraffin-embedded SARS-CoV-2 (COVID-19) Nucleocapsid FFPE Cell Pellet Block. Red: SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid stained by SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] (GTX632269) diluted at 1:1000. Green: SARS-CoV-2 (COVID-19) nucleocapsid stained by SARS-CoV-2 (COVID-19) nucleocapsid antibody (GTX135357) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920). Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX632269/GTX632269_43936_20200430_IHC-P-FL_Virus_2_w_23061202_770.webp "SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] detects SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid protein by immunohistochemical analysis. Sample: Paraffin-embedded SARS-CoV-2 (COVID-19) Nucleocapsid FFPE Cell Pellet Block. Red: SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid stained by SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] (GTX632269) diluted at 1:1000. Green: SARS-CoV-2 (COVID-19) nucleocapsid stained by SARS-CoV-2 (COVID-19) nucleocapsid antibody (GTX135357) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920). Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Non-infected (–) and infected (+, 48h pl MOI 0.01) Caco2 whole cell extracts were separated by SDS-PAGE, and the membrane was blotted with SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] (GTX632269) diluted at 1:1000.](https://www.genetex.com/upload/website/prouct_img/normal/GTX632269/GTX632269_42079_20200305_WB_SARScoronavirus_w_23061202_857.webp "Non-infected (–) and infected (+, 48h pl MOI 0.01) Caco2 whole cell extracts were separated by SDS-PAGE, and the membrane was blotted with SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] (GTX632269) diluted at 1:1000.")
![WB analysis of SARS-CoV infected Vero E6 cells harvested at different time-points post infection (T = 0, 8, 16 and 24 h) using GTX632269 SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3].](https://www.genetex.com/upload/website/prouct_img/normal/GTX632269/GTX632269_20200221_WB_w_23061202_994.webp "WB analysis of SARS-CoV infected Vero E6 cells harvested at different time-points post infection (T = 0, 8, 16 and 24 h) using GTX632269 SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3].")
SARS-CoV-2 (COVID-19) nucleocapsid protein (GTX135592-pro, 0.5 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] (GTX632269) diluted at 1:5000. The HRP-conjugated anti-mouset IgG antibody (GTX213111-01) was used to detect the primary antibody.
SARS-CoV / SARS-CoV-2 (COVID-19) Nucleocapsid antibody [6H3]
GTX632269
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityVirus
Overview
- SupplierGeneTex
- Product NameSARS-CoV / SARS-CoV-2 (COVID-19) Nucleocapsid antibody [6H3]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:1000-1:10000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID6H3
- Concentration1.5 mg/ml
- ConjugateUnconjugated
- HostMouse
- IsotypeIgG1
- ReactivityVirus
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Yang CF, Liao CC, Hsu HW, et al. Human ACE2 protein is a molecular switch controlling the mode of SARS-CoV-2 transmission. J Biomed Sci. 2023,30(1):87. doi: 10.1186/s12929-023-00980-wRead this paper
- Ma J, Wei J, Chen G, et al. Extracts of Thesium chinense inhibit SARS-CoV-2 and inflammation in vitro. Pharm Biol. 2023,61(1):1446-1453. doi: 10.1080/13880209.2023.2253841Read this paper
- Swain J, Merida P, Rubio K, et al. F-actin nanostructures rearrangements and regulation are essential for SARS-CoV-2 particle production in host pulmonary cells. iScience. 2023,26(8):107384. doi: 10.1016/j.isci.2023.107384Read this paper
- McMahon A, Andrews R, Groves D, et al. High-throughput super-resolution analysis of influenza virus pleomorphism reveals insights into viral spatial organization. PLoS Pathog. 2023,19(6):e1011484. doi: 10.1371/journal.ppat.1011484Read this paper
- Williams DM, Hornsby HR, Shehata OM, et al. Establishing SARS-CoV-2 membrane protein-specific antibodies as a valuable serological target via high-content microscopy. iScience. 2023,26(7):107056. doi: 10.1016/j.isci.2023.107056Read this paper
- Carrell C, Jang I, Link J, et al. Capillary driven microfluidic sequential flow device for point-of-need ELISA: COVID-19 serology testing. Anal Methods. 2023,15(22):2721-2728. doi: 10.1039/d3ay00225jRead this paper
- Yeh CT, Lee CY, Ho YJ, et al. Immunoglobulin Y Specific for SARS-CoV-2 Spike Protein Subunits Effectively Neutralizes SARS-CoV-2 Infectivity and Ameliorates Disease Manifestations In Vivo. Biomedicines. 2022,10(11). doi: 10.3390/biomedicines10112774Read this paper
- Tamai K, Sakai K, Yamaki H, et al. iPSC-derived mesenchymal cells that support alveolar organoid development. Cell Rep Methods. 2022,2(10):100314. doi: 10.1016/j.crmeth.2022.100314Read this paper
- Kumar CS, Singh B, Rizvi ZA, et al. Virus-Like Particles of SARS-CoV-2 as Virus Surrogates: Morphology, Immunogenicity, and Internalization in Neuronal Cells. ACS Infect Dis. 2022,8(10):2119-2132. doi: 10.1021/acsinfecdis.2c00217Read this paper
- Hou P, Wang X, Wang H, et al. The ORF7a protein of SARS-CoV-2 initiates autophagy and limits autophagosome-lysosome fusion via degradation of SNAP29 to promote virus replication. Autophagy. 2023,19(2):551-569. doi: 10.1080/15548627.2022.2084686Read this paper