Indirect ELISA analysis was performed by coating the plate with recombinant SARS-CoV-2 (COVID-19) Spike (ECD) Protein, Omicron / BA.4 / BA.5 variant, His tag (GTX137113-pro) (28.78-0.45 nM). Coated protein was probed with the specified SARS-CoV-2 (COVID-19) Spike S1 antibodies (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) or goat anti-mouse IgG antibody (HRP) (GTX213111-01) (1:10000) were used to detect the bound primary antibodies.
![Indirect ELISA analysis was performed by coating the plate with recombinant SARS-CoV-2 spike (ECD) trimer, omicron BA.2.75 variant, His tag (GTX137533-pro) (28.62-0.45 nM). Coated protein was probed with SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (the specified SARS-CoV-2 (COVID-19) Spike antibodies) (1 μg/mL). Goat anti-mouse IgG antibody (HRP) (GTX213111-01) (1:10000) was used to detect the bound primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX632604/GTX632604_44405_20220923_ELISA_Indirect_Multiple_22092622_834.webp "Indirect ELISA analysis was performed by coating the plate with recombinant SARS-CoV-2 spike (ECD) trimer, omicron BA.2.75 variant, His tag (GTX137533-pro) (28.62-0.45 nM). Coated protein was probed with SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (the specified SARS-CoV-2 (COVID-19) Spike antibodies) (1 μg/mL). Goat anti-mouse IgG antibody (HRP) (GTX213111-01) (1:10000) was used to detect the bound primary antibody.")
![Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike (ECD) protein, His tag (active) (GTX135972-pro) using antibodies as below. Capture: SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) (5 μg/mL) Detection: SARS-CoV-2 (COVID-19) Spike S2 antibody [HL1038] (GTX635910) (1 μg/mL)](https://www.genetex.com/upload/website/prouct_img/normal/GTX632604/GTX632604_44923_20220113_ELISA_PAIR_23011618_626.webp "Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike (ECD) protein, His tag (active) (GTX135972-pro) using antibodies as below. Capture: SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) (5 μg/mL) Detection: SARS-CoV-2 (COVID-19) Spike S2 antibody [HL1038] (GTX635910) (1 μg/mL)")
![SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] detects SARS-CoV-2 by immunofluorescent analysis. Sample: Vero E6 cells were infected with SARS-CoV-2 (MOI of 1) and fixed with chilled methanol/acetone. Green: SARS-CoV-2 infected Vero E6 cells stained by SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604). Blue: Nuclei were counterstained with DAPI. *From Zheng Z, et al. bioRxiv(2020). Shown under license agreement](https://www.genetex.com/upload/website/prouct_img/normal/GTX632604/GTX632604_20200430_ICC_IF_SARSCoronavirus2_w_23061202_809.webp "SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] detects SARS-CoV-2 by immunofluorescent analysis. Sample: Vero E6 cells were infected with SARS-CoV-2 (MOI of 1) and fixed with chilled methanol/acetone. Green: SARS-CoV-2 infected Vero E6 cells stained by SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604). Blue: Nuclei were counterstained with DAPI. *From Zheng Z, et al. bioRxiv(2020). Shown under license agreement")
![SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] detects SARS-CoV / SARS-CoV-2 (COVID-19) spike protein by immunohistochemical analysis. Sample: Paraffin-embedded SARS-CoV-2 (COVID-19) Spike FFPE Cell Pellet Block. Red: SARS-CoV / SARS-CoV-2 (COVID-19) spike stained by SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) diluted at 1:1000. Green: SARS-CoV-2 (COVID-19) spike stained by SARS-CoV-2 (COVID-19) spike antibody (GTX135356) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920). Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX632604/GTX632604_43937_20200430_IHC-P-FL_Virus_3_w_23061202_446.webp "SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] detects SARS-CoV / SARS-CoV-2 (COVID-19) spike protein by immunohistochemical analysis. Sample: Paraffin-embedded SARS-CoV-2 (COVID-19) Spike FFPE Cell Pellet Block. Red: SARS-CoV / SARS-CoV-2 (COVID-19) spike stained by SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) diluted at 1:1000. Green: SARS-CoV-2 (COVID-19) spike stained by SARS-CoV-2 (COVID-19) spike antibody (GTX135356) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920). Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Sandwich ELISA detection of recombinant SARS-CoV-2 spike (trimer) protein using antibodies as below.
Capture: SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) (5 μg/mL)
Detection: SARS-CoV-2 (COVID-19) Spike S1 antibody [HL13402] (GTX635713) (1 μg/mL).](https://www.genetex.com/upload/website/prouct_img/normal/GTX632604/GTX632604_44253_20210726_ELISA_PAIR_3_w_23061202_212.webp "Sandwich ELISA detection of recombinant SARS-CoV-2 spike (trimer) protein using antibodies as below.
Capture: SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) (5 μg/mL)
Detection: SARS-CoV-2 (COVID-19) Spike S1 antibody [HL13402] (GTX635713) (1 μg/mL).")
![Sandwich ELISA detection of recombinant full-length SARS-CoV-2 spike (trimer) protein using SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) as capture antibody at concentration of 5 μg/mL and SARS-CoV-2 (COVID-19) Spike RBD antibody (GTX135709) as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX632604/GTX632604_43957_20200821_ELISA_w_23061202_213.webp "Sandwich ELISA detection of recombinant full-length SARS-CoV-2 spike (trimer) protein using SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) as capture antibody at concentration of 5 μg/mL and SARS-CoV-2 (COVID-19) Spike RBD antibody (GTX135709) as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.")
Indirect ELISA analysis was performed by coating the plate with recombinant SARS-CoV-2 (COVID-19) Spike (ECD) Protein, Omicron / BA.4 / BA.5 variant, His tag (GTX137113-pro) (28.78-0.45 nM). Coated protein was probed with the specified SARS-CoV-2 (COVID-19) Spike S1 antibodies (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) or goat anti-mouse IgG antibody (HRP) (GTX213111-01) (1:10000) were used to detect the bound primary antibodies.
SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9]
GTX632604
ApplicationsElectron Microscopy, Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityVirus
Overview
- SupplierGeneTex
- Product NameSARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. ICC/IF: 1:100-1:2000. IHC-P: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsElectron Microscopy, Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID1A9
- Concentration1 mg/ml
- ConjugateUnconjugated
- HostMouse
- IsotypeIgG1
- ReactivityVirus
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
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- Dey D, Qing E, He Y, et al. A single C-terminal residue controls SARS-CoV-2 spike trafficking and incorporation into VLPs. Nat Commun. 2023,14(1):8358. doi: 10.1038/s41467-023-44076-3Read this paper
- Roessler J, Pich D, Krähling V, et al. SARS-CoV-2 and Epstein-Barr Virus-like Particles Associate and Fuse with Extracellular Vesicles in Virus Neutralization Tests. Biomedicines. 2023,11(11). doi: 10.3390/biomedicines11112892Read this paper
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- Lécuyer D, Nardacci R, Tannous D, et al. The purinergic receptor P2X7 and the NLRP3 inflammasome are druggable host factors required for SARS-CoV-2 infection. Front Immunol. 2023,14:1270081. doi: 10.3389/fimmu.2023.1270081Read this paper
- Carvalhal F, Magalhães AC, Rebelo R, et al. Evaluation of the Cytotoxic and Antiviral Effects of Small Molecules Selected by In Silico Studies as Inhibitors of SARS-CoV-2 Cell Entry. Molecules. 2023,28(20). doi: 10.3390/molecules28207204Read this paper
- Yang CF, Liao CC, Hsu HW, et al. Human ACE2 protein is a molecular switch controlling the mode of SARS-CoV-2 transmission. J Biomed Sci. 2023,30(1):87. doi: 10.1186/s12929-023-00980-wRead this paper