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SARS2 antibody [N3C2], Internal

GTX119451
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetSARS2
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Overview

  • Supplier
    GeneTex
  • Product Name
    SARS2 antibody [N3C2], Internal
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.97 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID54938
  • Target name
    SARS2
  • Target description
    seryl-tRNA synthetase 2, mitochondrial
  • Target synonyms
    mitochondrial seryl-tRNA synthetase; mtSerRS; SARS; SARSM; serine tRNA ligase 2, mitochondrial; serine--tRNA ligase, mitochondrial; SerRS; SerRSmt; SERS; seryl-tRNA synthetase, mitochondrial; seryl-tRNA(Ser/Sec) synthetase; SYS
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9NP81
  • Protein Name
    Serine--tRNA ligase, mitochondrial
  • Scientific Description
    This gene encodes the mitochondrial seryl-tRNA synthethase precursor, a member of the class II tRNA synthetase family. The mature enzyme catalyzes the ligation of Serine to tRNA(Ser) and participates in the biosynthesis of selenocysteinyl-tRNA(sec) in mitochondria. The enzyme contains an N-terminal tRNA binding domain and a core catalytic domain. It functions in a homodimeric form, which is stabilized by tRNA binding. This gene is regulated by a bidirectional promoter that also controls the expression of mitochondrial ribosomal protein S12. Both genes are within the critical interval for the autosomal dominant deafness locus DFNA4 and might be linked to this disease. Multiple transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SARS2 using anti-SARS2 antibody (A08425-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SARS2 antigen affinity purified polyclonal antibody (Catalog # A08425-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SARS2 at approximately 53 kDa. The expected band size for SARS2 is at 58 kDa.
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