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Anti-Scavenging Receptor SR-BI/SCARB1 Antibody

ORB308797
Biorbyt
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityMouse, Rat
TargetScarb1
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Overview

  • Supplier
    Biorbyt
  • Product Name
    Anti-Scavenging Receptor SR-BI/SCARB1 Antibody
  • Delivery Days Customer
    16
  • Application Supplier Note
    Western blot, 0.1-0.5microg/ml, Mouse, Rat Immunohistochemistry, 2-5 microg/ml, Rat. Add 0.2ml of distilled water will yield a concentration of 500ug/ml
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    IHC, WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    500 ug/ml
  • Conjugate
    Unconjugated
  • Gene ID20778
  • Target name
    Scarb1
  • Target description
    scavenger receptor class B, member 1
  • Target synonyms
    CD36, Cd36l1, Chohd1, Cla-1, Cla1, D5Ertd460e, Hdlq1, Hlb398, SR-B1, SR-BI, SRBI, Srb1, mSR-BI, scavenger receptor class B member 1, HDL QTL 1, scavenger receptor class B type I, scavenger receptor class B1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ61009
  • Protein Name
    Scavenger receptor class B member 1
  • Scientific Description
    Anti-Scavenging Receptor SR-BI/SCARB1 Antibody. Tested in IHC, WB applications. This antibody reacts with Mouse, Rat.
  • Reactivity
    Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SCARB1 using anti-SCARB1 antibody (PB9502). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SCARB1 antigen affinity purified polyclonal antibody (Catalog # PB9502) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SCARB1 at approximately 85 kDa. The expected band size for SCARB1 is at 57 kDa.
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