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ProductsBack/SCC112 (PDS5A) (C-term) Rabbit Polyclonal Antibody
Western blot analysis of SCC-112 / PDS5A Antibody (C-term) in A549 cell line lysates (35ug/lane). This demonstrates the PDS5A antibody detected the PDS5A protein (arrow).
Western blot analysis of SCC-112 / PDS5A Antibody (C-term) in A549 cell line lysates (35ug/lane). This demonstrates the PDS5A antibody detected the PDS5A protein (arrow).
Western blot analysis of SCC-112 / PDS5A Antibody (C-term) in A549 cell line lysates (35ug/lane). This demonstrates the PDS5A antibody detected the PDS5A protein (arrow).

SCC112 (PDS5A) (C-term) Rabbit Polyclonal Antibody

AP53248PU-N
OriGene
ApplicationsWestern Blot
Product group Antibodies
TargetPDS5A
400 ul
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Overview

  • Supplier
    OriGene
  • Product Name
    SCC112 (PDS5A) (C-term) Rabbit Polyclonal Antibody
  • Delivery Days Customer
    14
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID23244
  • Target name
    PDS5A
  • Target description
    PDS5 cohesin associated factor A
  • Target synonyms
    PIG54, SCC-112, SCC112, sister chromatid cohesion protein PDS5 homolog A, PDS5, regulator of cohesion maintenance, homolog A, cell proliferation-inducing gene 54 protein, sister chromatid cohesion protein 112
  • Host
    Rabbit
  • Protein IDQ29RF7
  • Protein Name
    Sister chromatid cohesion protein PDS5 homolog A
  • Scientific Description
    SCC112 (PDS5A) (C-term) rabbit polyclonal antibody, Aff - Purified
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of SCC112/PDS5A using anti-SCC112/PDS5A antibody (A06272-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: rat stomach tissue lysates, Lane 4: rat pancrease tissue lysates, Lane 5: mouse stomach tissue lysates, Lane 6: mouse pancrease tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SCC112/PDS5A antigen affinity purified polyclonal antibody (Catalog # A06272-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SCC112/PDS5A at approximately 151 kDa. The expected band size for SCC112/PDS5A is at 151 kDa.
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