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ProductsBack/SCD Polyclonal Antibody
Paraformaldehyde-fixed, paraffin embedded human liver cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with SCD1 Polyclonal Antibody (bs-3787R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining.
Paraformaldehyde-fixed, paraffin embedded human liver cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with SCD1 Polyclonal Antibody (bs-3787R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining.
Paraformaldehyde-fixed, paraffin embedded human liver cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with SCD1 Polyclonal Antibody (bs-3787R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining.

SCD Polyclonal Antibody

BS-3787R
Bioss Antibodies
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetSCD
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    SCD1 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID6319
  • Target name
    SCD
  • Target description
    stearoyl-CoA desaturase
  • Target synonyms
    acyl-CoA desaturase; delta(9)-desaturase; FADS5; fatty acid desaturase; hSCD1; MSTP008; predicted protein of HQ0998; SCD1; SCDOS; stearoyl-CoA desaturase; stearoyl-CoA desaturase (delta-9-desaturase); stearoyl-CoA desaturase opposite strand
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO00767
  • Protein Name
    Acyl-CoA desaturase
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • miR-760 exerts an antioncogenic effect in esophageal squamous cell carcinoma by negatively driving fat metabolism via targeting c-Myc. Yang X et al., 2020 Apr, J Cell Biochem
    Read more

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Figure 1. Western blot analysis of SCD using anti-SCD antibody (A00588-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SCD antigen affinity purified polyclonal antibody (Catalog # A00588-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SCD at approximately 37,42 kDa. The expected band size for SCD is at 42 kDa.
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