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SCG10 / STMN2 Antibody

LS-C408625
LifeSpan BioSciences
ApplicationsFlow Cytometry, Western Blot, ImmunoHistoChemistry
Product group Antibodies
TargetSTMN2
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    SCG10 / STMN2 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 20kDa/21kDa, while the observed MW by Western blot was 25kDa.
  • Applications
    Flow Cytometry, Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    Flo (1:20 - 1:50), IHC (1:50 - 1:100), WB (1:500 - 1:1000) The predicted MW is 20kDa/21kDa, while the observed MW by Western blot was 25kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID11075
  • Target name
    STMN2
  • Target description
    stathmin 2
  • Target synonyms
    SCG10, SCGN10, stathmin-2, neuron-specific growth-associated protein, neuronal growth-associated protein (silencer element), stathmin-like 2, superior cervical ganglia, neural specific 10, superior cervical ganglion-10 protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SCG10/STMN2 using anti-SCG10/STMN2 antibody (A04729-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: rat brain tissue lysates, Lane 3: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SCG10/STMN2 antigen affinity purified polyclonal antibody (Catalog # A04729-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SCG10/STMN2 at approximately 21KD. The expected band size for SCG10/STMN2 is at 21KD.
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