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ProductsBack/SCML1 antibody [N2C3]

SCML1 antibody [N2C3]

GTX114740
GeneTex
ApplicationsWestern Blot
DownloadDatasheet
Product group Antibodies
TargetSCML1
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Overview

  • Supplier
    GeneTex
  • Product Name
    SCML1 antibody [N2C3]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID6322
  • Target name
    SCML1
  • Target description
    Scm polycomb group protein like 1
  • Target synonyms
    sex comb on midleg like 1; sex comb on midleg-like protein 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UN30
  • Protein Name
    Sex comb on midleg-like protein 1
  • Scientific Description
    Putative Polycomb group (PcG) protein. PcG proteins act by forming multiprotein complexes, which are required to maintain the transcriptionally repressive state of homeotic genes throughout development. May be involved in spermatogenesis during sexual maturation.
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SCML1 using anti-SCML1 antibody (A12084-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SCML1 antigen affinity purified polyclonal antibody (Catalog # A12084-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SCML1 at approximately 37 kDa. The expected band size for SCML1 is at 37 kDa.
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