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Western Blot analysis of 293 cells using SCML2 Polyclonal Antibody
Western Blot analysis of 293 cells using SCML2 Polyclonal Antibody
Western Blot analysis of 293 cells using SCML2 Polyclonal Antibody

SCML2 Antibody

CSB-PA004749
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetSCML2
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Overview

  • Supplier
    Cusabio
  • Product Name
    SCML2 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10389
  • Target name
    SCML2
  • Target description
    Scm polycomb group protein like 2
  • Target synonyms
    sex comb on midleg like 2; sex comb on midleg-like protein 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UQR0
  • Protein Name
    Sex comb on midleg-like protein 2
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of SCML2 using anti-SCML2 antibody (A08958-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SCML2 antigen affinity purified polyclonal antibody (Catalog # A08958-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SCML2 at approximately 85 kDa. The expected band size for SCML2 is at 77 kDa.
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