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ProductsBack/SCN11A / Nav1.9 Antibody

SCN11A / Nav1.9 Antibody

LS-C402775
LifeSpan BioSciences
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
TargetSCN11A
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    SCN11A / Nav1.9 Antibody
  • Delivery Days Customer
    23
  • Applications
    ELISA, ImmunoHistoChemistry
  • Applications Supplier
    ELISA, IHC (1:50 - 1:200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.3 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID11280
  • Target name
    SCN11A
  • Target description
    sodium voltage-gated channel alpha subunit 11
  • Target synonyms
    FEPS3, HSAN7, NAV1.9, NaN, PN5, SCN12A, SNS-2, sodium channel protein type 11 subunit alpha, peripheral nerve sodium channel 5, sensory neuron sodium channel 2, sodium channel protein type XI subunit alpha, sodium channel, voltage-gated, type XI, alpha polypeptide, sodium channel, voltage-gated, type XI, alpha subunit, sodium channel, voltage-gated, type XII, alpha polypeptide, voltage-gated sodium channel subunit alpha Nav1.9
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SCN11A using anti-SCN11A antibody (A04126). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: rat brain tissue lysates, Lane 3: rat kidney tissue lysates, Lane 4: rat C6 whole cell lysates, Lane 5: mouse spleen tissue lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse kidney tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SCN11A antigen affinity purified polyclonal antibody (Catalog # A04126) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. Specific bands were detected for SCN11A at approximately 250KD. The expected band size for SCN11A is at 205KD.
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