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ProductsBack/SCN1A/SCN2A/SCN3A/SCN4A/SCN5A/SCN8A/SCN10A/SCN11A Antibody
Western Blot analysis of HuvEc cells using Na+ CP-pan Polyclonal Antibody
Western Blot analysis of HuvEc cells using Na+ CP-pan Polyclonal Antibody
Western Blot analysis of HuvEc cells using Na+ CP-pan Polyclonal Antibody

SCN1A/SCN2A/SCN3A/SCN4A/SCN5A/SCN8A/SCN10A/SCN11A Antibody

CSB-PA003363
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetSCN1A
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Overview

  • Supplier
    Cusabio
  • Product Name
    SCN1A/SCN2A/SCN3A/SCN4A/SCN5A/SCN8A/SCN10A/SCN11A Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6323
  • Target name
    SCN1A
  • Target description
    sodium voltage-gated channel alpha subunit 1
  • Target synonyms
    DEE6; DEE6A; DEE6B; DRVT; EIEE6; FEB3; FEB3A; FHM3; GEFSP2; HBSCI; NAC1; Nav1.1; SCN1; SMEI; sodium channel protein type 1 subunit alpha; sodium channel protein type I subunit alpha; sodium channel protein, brain I alpha subunit; sodium channel voltage gated type 1 alpha subunit; sodium channel, voltage-gated, type I, alpha polypeptide; sodium channel, voltage-gated, type I, alpha subunit; voltage-gated sodium channel subunit alpha Nav1.1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP35498
  • Protein Name
    Sodium channel protein type 1 subunit alpha
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Scn1a using anti-Scn1a antibody (PB9887). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates, Lane 3: U87 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Scn1a antigen affinity purified polyclonal antibody (Catalog # PB9887) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Scn1a at approximately 250 kDa. The expected band size for Scn1a is at 229 kDa.
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