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Western Blot analysis of HepG2 cells using Scn4b Polyclonal Antibody
Western Blot analysis of HepG2 cells using Scn4b Polyclonal Antibody
Western Blot analysis of HepG2 cells using Scn4b Polyclonal Antibody

SCN4B Antibody

CSB-PA004055
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetSCN4B
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Overview

  • Supplier
    Cusabio
  • Product Name
    SCN4B Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6330
  • Target name
    SCN4B
  • Target description
    sodium voltage-gated channel beta subunit 4
  • Target synonyms
    ATFB17, LQT10, Navbeta4, sodium channel regulatory subunit beta-4, sodium channel subunit beta-4, sodium channel, voltage-gated, type IV, beta subunit
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8IWT1
  • Protein Name
    Sodium channel subunit beta-4
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of SCN4B using anti-SCN4B antibody (A06453-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human U-87MG whole cell lysates, Lane 3: monkey COS-7 whole cell lysates, Lane 4: human U2OS whole cell lysates, Lane 5: human HEK293 whole cell lysates, Lane 6: human SHG-44 whole cell lysates, Lane 7: human K562 whole cell lysates (negative control), Lane 8: human HL-60 whole cell lysates (negative control). After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SCN4B antigen affinity purified polyclonal antibody (Catalog # A06453-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SCN4B at approximately 28KD. The expected band size for SCN4B is at 25KD.
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