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Immunohistochemistry of paraffin-embedded human heart tissue using CSB-PA020852LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human heart tissue using CSB-PA020852LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human heart tissue using CSB-PA020852LA01HU at dilution of 1:100

SCO1 Antibody

CSB-PA020852LA01HU
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
TargetSCO1
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Overview

  • Supplier
    Cusabio
  • Product Name
    SCO1 Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6341
  • Target name
    SCO1
  • Target description
    synthesis of cytochrome C oxidase 1
  • Target synonyms
    MC4DN4; protein SCO1 homolog, mitochondrial; SCO cytochrome c oxidase assembly protein 1; SCO cytochrome oxidase deficient homolog 1; SCO1, cytochrome c oxidase assembly protein; SCOD1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO75880
  • Protein Name
    Protein SCO1 homolog, mitochondrial
  • Scientific Description
    Thought to play a role in cellular copper homeostasis, mitochondrial redox signaling or insertion of copper into the active site of COX.
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Immunohistochemical staining of human cerebral cortex, colon, kidney and liver using Anti-SCO1 antibody HPA021579 (A) shows similar protein distribution across tissues to independent antibody HPA021565 (B).
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Figure 1. Western blot analysis of SCO1 using anti-SCO1 antibody (A02934-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Daudi whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SCO1 antigen affinity purified polyclonal antibody (Catalog # A02934-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SCO1 at approximately 30 kDa. The expected band size for SCO1 is at 34 kDa.
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