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ProductsBack/SCOP / PHLPP Antibody

SCOP / PHLPP Antibody

LS-C411076
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetPHLPP1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    SCOP / PHLPP Antibody
  • Delivery Days Customer
    23
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:500 - 1:2000)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID23239
  • Target name
    PHLPP1
  • Target description
    PH domain and leucine rich repeat protein phosphatase 1
  • Target synonyms
    PHLPP, PLEKHE1, PPM3A, SCOP, PH domain leucine-rich repeat-containing protein phosphatase 1, PH domain-containing family E member 1, SCN circadian oscillatory protein, pleckstrin homology domain containing, family E (with leucine rich repeats) member 1, protein phosphatase, Mg2+/Mn2+ dependent 3A, suprachiasmatic nucleus circadian oscillatory protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PHLPP1 using anti-PHLPP1 antibody (A02430-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A375 whole cell lysates, Lane 3: human Caco-2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PHLPP1 antigen affinity purified polyclonal antibody (Catalog # A02430-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PHLPP1 at approximately 140KD. The expected band size for PHLPP1 is at 185KD.
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