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WB analysis of mouse testis tissue using GTX15093 SCP3 antibody.
WB analysis of mouse testis tissue using GTX15093 SCP3 antibody.
WB analysis of mouse testis tissue using GTX15093 SCP3 antibody.

SCP3 antibody

GTX15093
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetSYCP3
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Overview

  • Supplier
    GeneTex
  • Product Name
    SCP3 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 0.5 microg/ml. ICC/IF: 1:100 - 1:500. IHC-P: 1:500 - 1:1500. IHC-Fr: 1:200 - 1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID50511
  • Target name
    SYCP3
  • Target description
    synaptonemal complex protein 3
  • Target synonyms
    COR1, RPRGL4, SCP3, SPGF4, synaptonemal complex protein 3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8IZU3
  • Protein Name
    Synaptonemal complex protein 3
  • Scientific Description
    This gene encodes an essential structural component of the synaptonemal complex. This complex is involved in synapsis, recombination and segregation of meiotic chromosomes. Mutations in this gene are associated with azoospermia in males and susceptibility to pregnancy loss in females. Alternate splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq]
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

References

  • Changes in Expressions of Spermatogenic Marker Genes and Spermatogenic Cell Population Caused by Stress. Tian P et al., 2021, Front Endocrinol (Lausanne)
    Read more

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Figure 1. Western blot analysis of SCP3/SYCP3 using anti-SCP3/SYCP3 antibody (A05718-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: rat testis tissue lysates, Lane 3: rat C6 whole cell lysates, Lane 4: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SCP3/SYCP3 antigen affinity purified polyclonal antibody (Catalog # A05718-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SCP3/SYCP3 at approximately 35 kDa. The expected band size for SCP3/SYCP3 is at 28 kDa.
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