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SDCCAG3 antibody [C1C3]

GTX106180
GeneTex
ApplicationsWestern Blot
DownloadDatasheet
Product group Antibodies
TargetENTR1
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Overview

  • Supplier
    GeneTex
  • Product Name
    SDCCAG3 antibody [C1C3]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID10807
  • Target name
    ENTR1
  • Target description
    endosome associated trafficking regulator 1
  • Target synonyms
    antigen NY-CO-3; endosome-associated-trafficking regulator 1; NY-CO-3; SDCCAG3; SDDAG3; serologically defined colon cancer antigen 3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96C92
  • Protein Name
    Serologically defined colon cancer antigen 3
  • Scientific Description
    May be involved in modulation of TNF response. May be involved in presentation of TNFRSF1A on the cell surface.
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ENTR1 using anti-ENTR1 antibody (A31716-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ENTR1 antigen affinity purified polyclonal antibody (Catalog # A31716-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ENTR1 at approximately 55 kDa. The expected band size for ENTR1 is at 48 kDa.
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