Various extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with SDF1 antibody [N1C3] (GTX116092) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
![SDF1 antibody [N1C3] detects SDF1 protein by immunofluorescent analysis. Sample: DIV9 rat cortical neuron and Glia cell cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SDF1 stained by SDF1 antibody [N1C3] (GTX116092) diluted at 1:50. Red: Tau, an axon marker, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.](https://www.genetex.com/upload/website/prouct_img/normal/GTX116092/GTX116092_44419_20211112_ICC_IF_R_w_23060519_321.webp "SDF1 antibody [N1C3] detects SDF1 protein by immunofluorescent analysis. Sample: DIV9 rat cortical neuron and Glia cell cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SDF1 stained by SDF1 antibody [N1C3] (GTX116092) diluted at 1:50. Red: Tau, an axon marker, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.")
![Whole cell extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with SDF1 antibody [N1C3] (GTX116092) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX116092/GTX116092_41724_20200918_WB_2_w_23060519_708.webp "Whole cell extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with SDF1 antibody [N1C3] (GTX116092) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
Various extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with SDF1 antibody [N1C3] (GTX116092) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
SDF1 antibody [N1C3]
GTX116092
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetCXCL12
Overview
- SupplierGeneTex
- Product NameSDF1 antibody [N1C3]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration0.16 mg/ml
- ConjugateUnconjugated
- Gene ID6387
- Target nameCXCL12
- Target descriptionC-X-C motif chemokine ligand 12
- Target synonymsIRH, PBSF, SCYB12, SDF1, TLSF, TPAR1, stromal cell-derived factor 1, chemokine (C-X-C motif) ligand 12, intercrine reduced in hepatomas, pre-B cell growth-stimulating factor
- HostRabbit
- IsotypeIgG
- Protein IDP48061
- Protein NameStromal cell-derived factor 1
- Scientific DescriptionFor background information on chemokines, see CXCL1 (MIM 155730). Stromal cell-derived factors 1-alpha and 1-beta are small cytokines that belong to the intercrine family, members of which activate leukocytes and are often induced by proinflammatory stimuli such as lipopolysaccharide, TNF (see MIM 191160), or IL1 (see MIM 147760). The intercrines are characterized by the presence of 4 conserved cysteines which form 2 disulfide bonds. They can be classified into 2 subfamilies. In the CC subfamily, which includes beta chemokine, the cysteine residues are adjacent to each other. In the CXC subfamily, which includes alpha chemokine, they are separated by an intervening amino acid. The SDF1 proteins belong to the latter group.[supplied by OMIM]
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Elaidy SM, El-Kherbetawy MK, Abed SY, et al. α-Hederin Saponin Augments the Chemopreventive Effect of Cisplatin against Ehrlich Tumors and Bioinformatic Approach Identifying the Role of SDF1/CXCR4/p-AKT-1/NFκB Signaling. Pharmaceuticals (Basel). 2023,16(3). doi: 10.3390/ph16030405Read this paper
- Leng M, Peng Y, Pan M, et al. Experimental Study on the Effect of Allogeneic Endothelial Progenitor Cells on Wound Healing in Diabetic Mice. J Diabetes Res. 2021,2021:9962877. doi: 10.1155/2021/9962877Read this paper
- Yang WY, Chen LC, Jhuang YT, et al. Injection of hybrid 3D spheroids composed of podocytes, mesenchymal stem cells, and vascular endothelial cells into the renal cortex improves kidney function and replenishes glomerular podocytes. Bioeng Transl Med. 2021,6(2):e10212. doi: 10.1002/btm2.10212Read this paper
- Hsu TW, Lu YJ, Lin YJ, et al. Transplantation of 3D MSC/HUVEC spheroids with neuroprotective and proangiogenic potentials ameliorates ischemic stroke brain injury. Biomaterials. 2021,272:120765. doi: 10.1016/j.biomaterials.2021.120765Read this paper
- Lin X, Wang H, Li X. Mobilization of endothelial progenitor cells promotes angiogenesis after full thickness excision by AMD3100 combined with G-CSF in diabetic mice by SDF-1/CXCR4 axis. Diab Vasc Dis Res. 2021,18(2):14791641211002473. doi: 10.1177/14791641211002473Read this paper
- Zhao J, An J, Liu S. Electroacupuncture at ST36 Increases Bone Marrow-Derived Interstitial Cells of Cajal via the SDF-1/CXCR4 and mSCF/Kit-ETV1 Pathways in the Stomach of Diabetic Mice. Evid Based Complement Alternat Med. 2018,2018:7878053. doi: 10.1155/2018/7878053Read this paper
- Feng Q, Guo P, Wang J, et al. High expression of SDF-1 and VEGF is associated with poor prognosis in patients with synovial sarcomas. Exp Ther Med. 2018,15(3):2597-2603. doi: 10.3892/etm.2018.5684Read this paper
Datasheet
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