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SDF2L1 Antibody

LS-C663323
LifeSpan BioSciences
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetSDF2L1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    SDF2L1 Antibody
  • Delivery Days Customer
    23
  • Applications
    ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
  • Applications Supplier
    ICC (5 - 20 µg/ml), IP, WB (0.2 - 2 µg/ml)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID23753
  • Target name
    SDF2L1
  • Target description
    stromal cell derived factor 2 like 1
  • Target synonyms
    stromal cell-derived factor 2-like protein 1, PWP1-interacting protein 8, SDF2-like 1, SDF2-like protein 1, dihydropyrimidinase-like 2
  • Host
    Rabbit
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of SDF2L1 using anti-SDF2L1 antibody (A13464). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: rat pancreas tissue lysates, Lane 6: rat testis tissue lysates, Lane 7: mouse pancreas tissue lysates, Lane 8: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SDF2L1 antigen affinity purified polyclonal antibody (Catalog # A13464) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SDF2L1 at approximately 24 kDa. The expected band size for SDF2L1 is at 24 kDa.
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