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AAV9 SaCas9 (78486) by BPS Bioscience. Order easily at Bio-Connect for fast, reliable delivery of research reagents across the Benelux region

78486 AAV9 SaCas9 - BPS Bioscience by Bio-Connect

AAV9 SaCas9

Adeno-Associated Virus serotype 9 (AAV9) is one of the most promising serotypes for gene therapy applications. AAV9 transduces a wide range of tissue types, including cardiac and skeletal muscle, and liver, pancreas, and eye tissue. AAV8 and AAV9 have recently been used to correct disease-causing mutations and improve muscle function in mouse models of Duchenne muscular dystrophy. AAV9 has significantly lower seroprevalence in the human population than other AAV serotypes, making AAV9 a desirable candidate for therapeutic applications. Cas9 is an endonuclease enzyme that introduces a double stranded break into the DNA when recruited to a specific DNA sequence by the sgRNA (single guide RNA). This double stranded break is repaired in mammalian cells either through Non-Homologous End Joining or Homologous Recombination. Non-Homologous End Joining often results in the deletion or insertion of several base pairs at the cut site, which, when resulting in a frameshift, causes the functional inactivation of the gene. SaCas9 (Staphylococcus aureus CRISPR-associated protein 9) has demonstrated high cutting efficiency in mammalian cells, and its smaller size makes it ideal for packaging into AAV. SaCas9 recognizes a longer protospacer adjacent motif (PAM) site, 5-NNGRRT-3, than the more traditional SpCas9 (Streptococcus pyogenes CRISPR-associated protein 9). These AAV particles constitutively express SaCas9 under the control of a CMV promoter.

Enhance your research with AAV qPCR Titration Kit (82812), proven in Other by BPS Bioscience. Preferred supplier for academia & industry

82812 AAV qPCR Titration Kit - BPS Bioscience by Bio-Connect

Luciferase, His-Tag (Firefly) Recombinant

The AAV qPCR Titration Kit is a kit designed to measure AAV (adeno-associated virus) titers of particles with AAV2 ITR (inverted terminal repeats) by qPCR (quantitative polymerase chain-reaction). It offers a streamlined, one-step process without needing digestion steps. Engineered for high sensitivity and specificity, it minimizes non-specific background, enhancing performance over comparable kits. The kit contains enough reagents for 100 reactions. It includes a separate ROX (carboxyrhodamine), a passive fluorescence reference dye that allows for signal normalization, and an AAV reference material as qPCR standard for precise quantification. This product has been validated across multiple AAV samples with varying serotypes, purification status, and encapsulated transgenes, consistently demonstrating high levels of accuracy, precision, and robustness. Need custom AAV design and production? Visit our AAV service page for more information.

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