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Search results: AP2M1

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Product group Proteins / Signaling Molecules
Atlas Antibodies
Protein IDQ96CW1
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Product group Proteins / Signaling Molecules
Atlas Antibodies
Protein IDQ96CW1
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Product group Antibodies
Assay Genie
ReactivityHuman
TargetEPCAM
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Product group Antibodies
Bioss Antibodies
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
ReactivityChicken, Human, Mouse, Rat, Sheep
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Product group Antibodies
Cusabio
ApplicationsELISA
ReactivityHuman
TargetAP2M1
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Product group Antibodies
Cusabio
ReactivityHuman
TargetAP2M1
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Product group Antibodies
Cusabio
ApplicationsELISA
ReactivityHuman
TargetAP2M1
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Product group Antibodies
Cusabio
ApplicationsELISA
ReactivityHuman
TargetAP2M1
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Product group Antibodies
Cusabio
ReactivityHuman
TargetAP2M1
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Product group Antibodies
Cusabio
ApplicationsELISA
ReactivityHuman
TargetAP2M1
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Figure 1. Western blot analysis of AP2M1 using anti-AP2M1 antibody (A06179). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human U2OS whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: rat kidney tissue lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AP2M1 antigen affinity purified polyclonal antibody (Catalog # A06179) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AP2M1 at approximately 50KD. The expected band size for AP2M1 is at 50KD.
Product group Antibodies
Boster Bio
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetAP2M1
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Figure 1. Western blot analysis of AP2M1 using anti-AP2M1 antibody (A06179). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human U2OS whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: rat kidney tissue lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AP2M1 antigen affinity purified polyclonal antibody (Catalog # A06179) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AP2M1 at approximately 50KD. The expected band size for AP2M1 is at 50KD.
Product group Antibodies
Boster Bio
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetAP2M1
  • SizePrice